PMID- 18953051
OWN - NLM
STAT- MEDLINE
DCOM- 20090202
LR  - 20081202
IS  - 1522-9645 (Electronic)
IS  - 0195-668X (Linking)
VI  - 29
IP  - 23
DP  - 2008 Dec
TI  - Bone marrow cells are a rich source of growth factors and cytokines: implications 
      for cell therapy trials after myocardial infarction.
PG  - 2851-8
LID - 10.1093/eurheartj/ehn456 [doi]
AB  - AIMS: Results from clinical trials suggest that cardiac function after acute 
      myocardial infarction (AMI) can be enhanced by an intracoronary infusion of 
      autologous unselected nucleated bone marrow cells (BMCs). Release of paracrine 
      factors has been proposed as a mechanism for these therapeutic effects; however, 
      this hypothesis has not been tested in humans. METHODS AND RESULTS: BMCs and 
      peripheral blood leucocytes (PBLs) were obtained from 15 patients with AMI and 
      cultured in serum-free medium to obtain conditioned supernatants (SN). BMC-SN 
      stimulated human coronary artery endothelial cell proliferation, migration, and 
      tube formation, and induced cell sprouting in a mouse aortic ring assay. 
      Moreover, BMC-SN protected rat cardiomyocytes from cell death induced by 
      simulated ischaemia or ischaemia followed by reperfusion. While PBL-SN promoted 
      similar effects on endothelial cells and cardiomyocytes, BMC-SN and PBL-SN in 
      combination promoted synergistic effects. As shown by ProteinChip and GeneChip 
      array analyses (each performed in triplicate), BMCs and PBLs expressed distinct 
      patterns of pro-angiogenic and cytoprotective secreted factors. CONCLUSION: Our 
      data support the paracrine hypothesis and suggest that characterization of the 
      BMC secretome may lead to an identification of factors with therapeutic potential 
      after AMI.
FAU - Korf-Klingebiel, Mortimer
AU  - Korf-Klingebiel M
AD  - Department of Cardiology and Angiology, Hannover Medical School, 
      Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
FAU - Kempf, Tibor
AU  - Kempf T
FAU - Sauer, Thomas
AU  - Sauer T
FAU - Brinkmann, Eva
AU  - Brinkmann E
FAU - Fischer, Philipp
AU  - Fischer P
FAU - Meyer, Gerd P
AU  - Meyer GP
FAU - Ganser, Arnold
AU  - Ganser A
FAU - Drexler, Helmut
AU  - Drexler H
FAU - Wollert, Kai C
AU  - Wollert KC
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20081025
PL  - England
TA  - Eur Heart J
JT  - European heart journal
JID - 8006263
RN  - 0 (Cytokines)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*metabolism
MH  - Bone Marrow Transplantation/*methods
MH  - Coronary Vessels/physiology/*surgery
MH  - Cytokines/administration & dosage/*metabolism
MH  - Female
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins
MH  - Male
MH  - Mice
MH  - Middle Aged
MH  - Myocardial Infarction/*therapy
MH  - Myocytes, Cardiac/metabolism
MH  - Treatment Outcome
EDAT- 2008/10/28 09:00
MHDA- 2009/02/03 09:00
CRDT- 2008/10/28 09:00
PHST- 2008/10/28 09:00 [pubmed]
PHST- 2009/02/03 09:00 [medline]
PHST- 2008/10/28 09:00 [entrez]
AID - ehn456 [pii]
AID - 10.1093/eurheartj/ehn456 [doi]
PST - ppublish
SO  - Eur Heart J. 2008 Dec;29(23):2851-8. doi: 10.1093/eurheartj/ehn456. Epub 2008 Oct 
      25.