PMID- 18953722
OWN - NLM
STAT- MEDLINE
DCOM- 20081117
LR  - 20191111
IS  - 1431-6730 (Print)
IS  - 1431-6730 (Linking)
VI  - 389
IP  - 5
DP  - 2008 May
TI  - Analysis of the human cytomegalovirus pp65-directed T-cell response in healthy 
      HLA-A2-positive individuals.
PG  - 551-9
AB  - Human cytomegalovirus (HCMV) is contained by T-lymphocyte responses focused 
      towards the major tegument protein pp65. To systematically identify T-cell 
      epitopes, we applied the following strategy: 441 overlapping 15mer peptides 
      spanning the entire HCMV pp65 antigen in 1-aa steps were screened in 
      enzyme-linked immunospot (ELispot) assays for interferon gamma (IFN-gamma) 
      secretion by peripheral blood mononuclear cells (PBMCs) from nine healthy 
      HCMV-seropositive subjects expressing human leukocyte antigen (HLA)-A2. This 
      analysis confirmed a number of previously known epitopes and revealed several new 
      ones. A total of 26 epitopes were identified, including 14 HLA-A2, four HLA-B7, 
      -B35, -812 and -B44 restricted class I epitopes, six class II epitopes, and two 
      epitopes of unknown restriction. Three novel HLA-A2 epitopes were confirmed using 
      T2-cells, and one peptide for which only binding data had been published so far 
      was verified. Two novel class II epitopes were confirmed by intracellular 
      cytokine staining. Responses were usually oligoclonal against up to seven HLA-A2 
      epitopes, albeit with a few dominating epitopes. Clusters of overlapping epitopes 
      (hot-spots) were identified. These and the newly identified T-cell epitopes may 
      be of great value for epitope-based immunotherapeutic approaches, including 
      peptide vaccines.
FAU - Schalich, Juliane
AU  - Schalich J
AD  - InterCell AG, Campus Vienna Biocenter 6, A-1030 Vienna, Austria.
FAU - Vytvytska, Oresta
AU  - Vytvytska O
FAU - Zauner, Wolfgang
AU  - Zauner W
FAU - Fischer, Michael B
AU  - Fischer MB
FAU - Buschle, Michael
AU  - Buschle M
FAU - Aichinger, Gerald
AU  - Aichinger G
FAU - Klade, Christoph S
AU  - Klade CS
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Biol Chem
JT  - Biological chemistry
JID - 9700112
RN  - 0 (Cytomegalovirus Vaccines)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Peptide Fragments)
RN  - 0 (Phosphoproteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (cytomegalovirus matrix protein 65kDa)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cytomegalovirus/*immunology
MH  - Cytomegalovirus Vaccines/chemical synthesis/*immunology
MH  - Drug Evaluation, Preclinical
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - Flow Cytometry
MH  - HLA-A2 Antigen/*immunology
MH  - Humans
MH  - Interferon-gamma/metabolism/pharmacology
MH  - Molecular Sequence Data
MH  - Monocytes/immunology
MH  - Peptide Fragments/chemical synthesis/immunology
MH  - Phosphoproteins/*immunology
MH  - T-Lymphocytes/*immunology
MH  - Viral Matrix Proteins/*immunology
EDAT- 2008/10/28 09:00
MHDA- 2008/11/18 09:00
CRDT- 2008/10/28 09:00
PHST- 2008/10/28 09:00 [pubmed]
PHST- 2008/11/18 09:00 [medline]
PHST- 2008/10/28 09:00 [entrez]
AID - 10.1515/bc.2008.065 [doi]
PST - ppublish
SO  - Biol Chem. 2008 May;389(5):551-9. doi: 10.1515/bc.2008.065.