PMID- 18953909
OWN - NLM
STAT- MEDLINE
DCOM- 20110310
LR  - 20081028
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 43
IP  - 5
DP  - 2008 May
TI  - [Monocyte chemoattractant protein-1 regulations of human gingival fibroblasts by 
      Porphyromonas gingivalis with different fimA genotypes].
PG  - 269-72
AB  - OBJECTIVE: To investigate the mechanism of monocyte chemoattractant protein-1 
      (MCP-1) regulations of human gingival fibroblasts (HGF) by challenge of 
      Porphyromonas gingivalis (Pg) with different fimA genotypes. METHODS: Pg ATCC 
      33277 (type I), WCSP115 (type II), WCSP1.5 (type III), W83 (type IV) were 
      assessed for their inductions of MCP-1 expression in HGF. MCP-1 mRNA levels of 
      HGF were determined by real-time RT-PCR and MCP-1 protein levels in culture 
      supernatant by ELISA at different time intervals (1 h, 3h, 6h and 12h) following 
      continuous co-culture of bacteria with HGF. RESULTS: MCP-1 mRNA and protein 
      levels were both up-regulated when HGF co-cultured with different Pg fimA 
      genotypes. Type II was stronger than other fimA genotypes, HGF expressed 
      significantly great amount of MCP-1 mRNA [(25.75 +/- 3.12)-(326.69 +/- 35.35)] 
      and protein [(178.20 +/- 46.20)-(443.46 82.19) ng/L] for different time periods; 
      While Type III was weaker than other fimA genotypes, and the level of MCP-1 mRNA 
      was [ (4.16 +/- 0.82)-(94.17 +/- 18.56)] and protein [(86.95 +/- 23.90)-(264.01 
      +/- 28.59) ng/L](P < 0.05). CONCLUSIONS: fimA genotypes of Pg are related with 
      the inductions of MCP-1, which might indicate fimA genotype is associated with 
      pathogenesis of Pg.
FAU - Zhao, Lei
AU  - Zhao L
AD  - State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan 
      University, Chengdu 610041, China.
FAU - Wu, Ya-fei
AU  - Wu YF
FAU - Ouyang, Yu-ling
AU  - Ouyang YL
FAU - Zhang, Jing-yi
AU  - Zhang JY
FAU - Chen, Bin
AU  - Chen B
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (fimbrillin)
RN  - 147680-16-8 (Fimbriae Proteins)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Fibroblasts/*metabolism
MH  - Fimbriae Proteins/*genetics
MH  - Genotype
MH  - Gingiva/cytology/*microbiology
MH  - Humans
MH  - Porphyromonas gingivalis/*genetics
EDAT- 2008/10/29 09:00
MHDA- 2011/03/11 06:00
CRDT- 2008/10/29 09:00
PHST- 2008/10/29 09:00 [pubmed]
PHST- 2011/03/11 06:00 [medline]
PHST- 2008/10/29 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2008 May;43(5):269-72.