PMID- 18953944
OWN - NLM
STAT- MEDLINE
DCOM- 20090303
LR  - 20140226
IS  - 0578-1426 (Print)
IS  - 0578-1426 (Linking)
VI  - 47
IP  - 5
DP  - 2008 May
TI  - [The plasmic translocation and release of high mobility group box chromosomal 
      protein 1 in peripheral blood monocytes of patients with rheumatoid arthritis and 
      the effect of thalidomide].
PG  - 374-7
AB  - OBJECTIVE: To investigate the release and intracellular localization of high 
      mobility group box chromosomal protein 1 (HMGB1) in the peripheral blood 
      monocytes of rheumatoid arthritis (RA) patients and the inhibitive effect of 
      thalidomide. METHODS: 19 RA patients and 20 healthy controls were included in the 
      study. Monocytes were separated from peripheral blood with Ficoll density 
      gradient centrifugation. Monocytes were treated with 100 ng/ml tumor necrosis 
      factor alpha (TNFalpha) or 100 ng/ml TNFalpha plus 40 microg/ml thalidomide and 
      grown in an incubator at 37 degrees C with 5% CO2 for 24 hours. The culture 
      supernatants of the monocytes were collected. HMGB1 level in the culture medium 
      was detected with Western blot. In addition, the intracellular localization of 
      HMGB1 in the monocytes was investigated with immunocytochemical analysis. 
      RESULTS: Without stimulation, the release of HMGB1 protein was significantly 
      increased in the culture supernatants of peripheral blood monocytes from RA 
      patients as compared with that from healthy controls (P < 0.05). TNFalpha (100 
      ng/ml) did not further increase the release of HMGB1 in the monocytes from the 
      patients with RA. Thalidomide (40 microg/ml) could inhibit the release of HMGB1 
      in the monocytes from RA patients stimulated with TNFalpha (P < 0.05). In the 
      monocytes from RA patients, HMGB1 was mainly localized in the nucleus. Treatment 
      with TNFalpha (100 ng/ml) for 24 hours resulted in a cytoplasmic translocation of 
      HMGB1, which was inhibited significantly by thalidomide. CONCLUSION: TNFalpha 
      induces the release and cytoplasmic translocation of HMGB1 in the peripheral 
      blood monocytes of RA patients and thalidomide inhibits the release and 
      translocation of HMGB1.
FAU - Zuo, Xiao-Xia
AU  - Zuo XX
AD  - Department of Rheumatology and Immunology, Xiang-ya Hospital, Central South 
      University, Changsha 410008, China. susanzuo@hotmail.com
FAU - Gong, Yan-Hui
AU  - Gong YH
FAU - Zhou, Ya-Ou
AU  - Zhou YO
FAU - Luo, Hui
AU  - Luo H
FAU - Xiao, Xian-Zhong
AU  - Xiao XZ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Nei Ke Za Zhi
JT  - Zhonghua nei ke za zhi
JID - 16210490R
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (HMGB1 Protein)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4Z8R6ORS6L (Thalidomide)
SB  - IM
MH  - Adult
MH  - Arthritis, Rheumatoid/*blood/pathology
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Culture
MH  - Culture Media, Conditioned/chemistry/metabolism
MH  - Female
MH  - HMGB1 Protein/*metabolism
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Monocytes/cytology/*drug effects/metabolism
MH  - Thalidomide/*pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2008/10/29 09:00
MHDA- 2009/03/04 09:00
CRDT- 2008/10/29 09:00
PHST- 2008/10/29 09:00 [pubmed]
PHST- 2009/03/04 09:00 [medline]
PHST- 2008/10/29 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Nei Ke Za Zhi. 2008 May;47(5):374-7.