PMID- 18954501
OWN - NLM
STAT- MEDLINE
DCOM- 20081114
LR  - 20081028
IS  - 1050-6586 (Print)
IS  - 1050-6586 (Linking)
VI  - 22
IP  - 5
DP  - 2008 Sep-Oct
TI  - Antigen-specific IgE in sinus mucosa of allergic fungal rhinosinusitis patients.
PG  - 451-6
LID - 10.2500/ajr.2008.22.3227 [doi]
AB  - BACKGROUND: Local tissue production of antigen-specific immunoglobulin E (IgE) 
      has been shown in patients with allergic rhinitis and in patients with chronic 
      rhinosinusitis (CRS) with nasal polyps. In allergic fungal rhinosinusitis (AFRS), 
      specific IgE has been established in nasal lavage fluid and eosinophilic mucin. 
      In this study, local production of antigen-specific IgE within sinus mucosa of 
      AFRS patients was evaluated. METHODS: Sinus mucosa homogenates from 11 AFRS 
      patients, 8 patients with CRS without nasal polyps (CRSsNP), and 9 
      nonrhinosinusitis control patients were assessed for IgE localization by 
      immunohistochemistry. AFRS and control tissue homogenates were also evaluated for 
      antigen-specific IgE to 14 common antigens by ImmunoCAP testing (Phadia AB, 
      Portage, MI). RESULTS: There was a significant increase in IgE staining in AFRS 
      sinus epithelium and subepithelium compared with controls and with patients with 
      CRSsNP (p <or= 0.012 for all group differences). AFRS patients showed increased 
      IgE staining in the subepithelium when compared with epithelium (p < 0.001). AFRS 
      sinus tissue had significantly more IgE measured by ImmunoCAP when compared with 
      control sinus tissue for 7 of 14 specific antigens (p < 0.05) and for total IgE 
      (p = 0.004). Antigens with a significant difference on ImmunoCAP included 
      Cladosporium, Aspergillus, Timothy grass, red maple, cockroach, ragweed, and 
      cocklebur. CONCLUSION: AFRS patients showed significantly more IgE in sinus 
      mucosa tissue specimens, with increased IgE in subepithelial sites when compared 
      with epithelium. The increased expression of antigen-specific IgE is not limited 
      to fungal antigens. These findings support the role of type I hypersensitivity 
      and local manifestations of allergy in AFRS patients.
FAU - Wise, Sarah K
AU  - Wise SK
AD  - Department of Otolaryngology-Head and Neck Surgery, Medical University of South 
      Carolina, Charleston, South Carolina 29425, USA.
FAU - Ahn, Chadwick N
AU  - Ahn CN
FAU - Lathers, Deanne M R
AU  - Lathers DM
FAU - Mulligan, Ryan M
AU  - Mulligan RM
FAU - Schlosser, Rodney J
AU  - Schlosser RJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Rhinol
JT  - American journal of rhinology
JID - 8807268
RN  - 0 (Antigens, Fungal)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antigens, Fungal/*biosynthesis/immunology
MH  - Cell Count
MH  - Chronic Disease
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Mycoses/*immunology/microbiology/pathology
MH  - Paranasal Sinuses/*immunology/microbiology/pathology
MH  - Photomicrography
MH  - Rhinitis, Allergic, Perennial/*immunology/microbiology/pathology
MH  - Sinusitis/*immunology/microbiology/pathology
EDAT- 2008/10/29 09:00
MHDA- 2008/11/15 09:00
CRDT- 2008/10/29 09:00
PHST- 2008/10/29 09:00 [pubmed]
PHST- 2008/11/15 09:00 [medline]
PHST- 2008/10/29 09:00 [entrez]
AID - 10.2500/ajr.2008.22.3227 [doi]
PST - ppublish
SO  - Am J Rhinol. 2008 Sep-Oct;22(5):451-6. doi: 10.2500/ajr.2008.22.3227.