PMID- 18954504
OWN - NLM
STAT- MEDLINE
DCOM- 20081114
LR  - 20111117
IS  - 1050-6586 (Print)
IS  - 1050-6586 (Linking)
VI  - 22
IP  - 5
DP  - 2008 Sep-Oct
TI  - Decreased production of human leukocyte antigen G molecules in sinonasal 
      polyposis.
PG  - 468-73
LID - 10.2500/ajr.2008.22.3210 [doi]
AB  - BACKGROUND: Sinonasal polyposis (SNP) is a chronic inflammatory pathology of 
      nasal and paranasal cavities. Human leukocyte antigen (HLA) G molecules are 
      nonclassic class I antigens with anti-inflammatory and tolerogenic properties. As 
      most theories consider polyps to be the manifestation of chronic inflammation, 
      there could be a possible implication of HLA-G molecules in SNP. The purpose of 
      this study was to investigate the possible correlation between SNP and the 
      production of soluble HLA-G (sHLA-G) by peripheral blood mononuclear cells 
      (PBMCs). METHODS: The study involved 22 SNP patients (11 with no evidence of 
      disease [NED] after surgery and 11 with relapse [RE]) and 20 healthy subjects. 
      The presence of sHLA-G in PBMC lipopolysaccharide (LPS)-stimulated culture 
      supernatants was analyzed. The levels of interleukin (IL) 10, one of the main 
      up-regulators of sHLA-G production, were determined. Exogenous IL-10 was added to 
      the SNP PBMC cultures to reconstitute the impairment in sHLA-G production. 
      RESULTS: Increased IL-10 levels in LPS-activated PBMC culture supernatants were 
      found in NED patients in comparison with healthy subjects (p = 0.0184). No sHLA-G 
      production was observed in either of the patient subgroup supernatants (p < 
      0.0001). The addition of exogenous IL-10 showed the reconstitution of sHLA-G 
      production in NED and in a lower amount in RE patients. CONCLUSION: The results 
      show a defect in sHLA-G production in SNP patients mainly related to the 
      IL-10/HLA-G pathway. Given the anti-inflammatory functions of HLA-G molecules, 
      this impairment could increase the susceptibility to the disease. The different 
      sHLA-G production after exogenous IL-10 addition between NED and RE SNP could 
      represent a marker of disease severity.
FAU - Malagutti, Nicola
AU  - Malagutti N
AD  - Department of Ear, Nose, and Throat, University of Ferrara, Ferrara, Italy.
FAU - Aimoni, Claudia
AU  - Aimoni C
FAU - Balboni, Alessandra
AU  - Balboni A
FAU - Stignani, Marina
AU  - Stignani M
FAU - Melchiorri, Loredana
AU  - Melchiorri L
FAU - Borin, Michela
AU  - Borin M
FAU - Pastore, Antonio
AU  - Pastore A
FAU - Rizzo, Roberta
AU  - Rizzo R
FAU - Baricordi, Olavio Roberto
AU  - Baricordi OR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Am J Rhinol
JT  - American journal of rhinology
JID - 8807268
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Cells, Cultured
MH  - Female
MH  - Flow Cytometry
MH  - Follow-Up Studies
MH  - HLA Antigens/*biosynthesis/immunology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*biosynthesis/immunology
MH  - Humans
MH  - Immunoassay
MH  - Interleukin-10/biosynthesis
MH  - Leukocytes, Mononuclear/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Nasal Polyps/*immunology/metabolism/pathology
MH  - Paranasal Sinus Diseases/*immunology/metabolism/pathology
MH  - Retrospective Studies
MH  - Severity of Illness Index
EDAT- 2008/10/29 09:00
MHDA- 2008/11/15 09:00
CRDT- 2008/10/29 09:00
PHST- 2008/10/29 09:00 [pubmed]
PHST- 2008/11/15 09:00 [medline]
PHST- 2008/10/29 09:00 [entrez]
AID - 10.2500/ajr.2008.22.3210 [doi]
PST - ppublish
SO  - Am J Rhinol. 2008 Sep-Oct;22(5):468-73. doi: 10.2500/ajr.2008.22.3210.