PMID- 18956727
OWN - NLM
STAT- MEDLINE
DCOM- 20090421
LR  - 20181201
IS  - 0529-5815 (Print)
IS  - 0529-5815 (Linking)
VI  - 46
IP  - 9
DP  - 2008 May 1
TI  - [Differentiation of human telomerase reverse transcriptase immortalized human 
      marrow mesenchymal stem cell into chondrocyte].
PG  - 697-9
AB  - OBJECTIVE: To establish an immortalized marrow mesenchymal stem cell line to 
      facilitate advances in cartilage engineering research. METHODS: Human telomerase 
      reverse transcriptase (hTERT) cDNA was transferred into primary human marrow 
      mesenchymal stem cells (hMSC) by retroviral vector pLEGFP-C1-hTERT. Subsequently 
      G418 resistant cell clone was screened and expanded for further studies. hMSC 
      biomarkers and hTERT expression were confirmed by examination. Transfected hMSC 
      was induced to differentiate into chondrocyte using TGF-P1 and dexamethasone. 
      RESULTS: Up-regulated hTERT expression was detected in transfected hMSC. 
      hMSC-hTERT cells could be induced to differentiate into chondrocyte. Higher 
      telomerase activity in transfected cells was maintained for 50 population 
      doublings so far. Collagen II could be detected in induced transfected hMSC by 
      immunocytochemical and hybridization in situ. CONCLUSIONS: Ectopic expression of 
      hTERT can effectively immortalize hMSC in vitro. Immortalized hMSC can be induced 
      to differentiate into chondrocyte under certain condition. It may be an ideal 
      target of further studies in cartilage engineering.
FAU - Qi, Zhi-Ming
AU  - Qi ZM
AD  - Department of Orthopaedics, the First Affiliated Hospital of Medical University 
      of China, Shenyang 110001, China. qzhming@yahoo.com.cn
FAU - Lu, Gang
AU  - Lu G
FAU - Bai, Yan-Dong
AU  - Bai YD
FAU - Wang, Hong
AU  - Wang H
FAU - Wang, Ling
AU  - Wang L
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Wai Ke Za Zhi
JT  - Zhonghua wai ke za zhi [Chinese journal of surgery]
JID - 0153611
RN  - EC 2.7.7.49 (TERT protein, human)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Bone Marrow Cells/*cytology/drug effects/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Line, Transformed
MH  - Chondrocytes/*cytology
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - Telomerase/*genetics/metabolism
MH  - Transfection
EDAT- 2008/10/30 09:00
MHDA- 2009/04/22 09:00
CRDT- 2008/10/30 09:00
PHST- 2008/10/30 09:00 [pubmed]
PHST- 2009/04/22 09:00 [medline]
PHST- 2008/10/30 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Wai Ke Za Zhi. 2008 May 1;46(9):697-9.