PMID- 18957483
OWN - NLM
STAT- MEDLINE
DCOM- 20090427
LR  - 20220224
IS  - 1468-2060 (Electronic)
IS  - 0003-4967 (Linking)
VI  - 68
IP  - 4
DP  - 2009 Apr
TI  - New autoantigens in rheumatoid arthritis (RA): screening 8268 protein arrays with 
      sera from patients with RA.
PG  - 591-4
LID - 10.1136/ard.2008.096917 [doi]
AB  - OBJECTIVE: To identify new IgG autoantibodies in sera from patients with 
      rheumatoid arthritis (RA). METHODS: We tested serum samples from 19 patients with 
      RA with given human leukocyte antigen (HLA)-DR genotypes, from 7 patients with 
      spondylarthropathy, 2 patients with lupus, 4 patients with systemic sclerosis and 
      10 healthy individuals on 8268 human protein arrays. RESULTS: We identified four 
      antigens (peptidyl arginine deiminase 4 (PAD4), protein kinase Cbeta1 (PKCbeta1), 
      phosphatylinositol 4 phosphate 5 kinase type II gamma (PIP4K2C) and v raf murine 
      sarcoma viral oncogene homologue B1 catalytic domain (BRAF)) that were recognised 
      almost uniquely by sera from patients with RA on protein arrays. Using purified 
      proteins, we confirmed that PAD4 and BRAF are recognised almost uniquely by 
      patients with RA. CONCLUSION: We identified PAD4 and BRAF as RA specific 
      autoantigens.
FAU - Auger, I
AU  - Auger I
AD  - INSERM UMR 639, Parc Scientifique de Luminy, APHM La Conception, Marseille, 
      France. isabelle.auger@medecine.univ-mrs.fr
FAU - Balandraud, N
AU  - Balandraud N
FAU - Rak, J
AU  - Rak J
FAU - Lambert, N
AU  - Lambert N
FAU - Martin, M
AU  - Martin M
FAU - Roudier, J
AU  - Roudier J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081028
PL  - England
TA  - Ann Rheum Dis
JT  - Annals of the rheumatic diseases
JID - 0372355
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (Immunoglobulin G)
RN  - EC 2.7.11.1 (BRAF protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins B-raf)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (Protein Kinase C beta)
RN  - EC 3.- (Hydrolases)
RN  - EC 3.5.3.15 (PADI4 protein, human)
RN  - EC 3.5.3.15 (Protein-Arginine Deiminase Type 4)
RN  - EC 3.5.3.15 (Protein-Arginine Deiminases)
SB  - IM
MH  - Arthritis, Rheumatoid/*immunology
MH  - Autoantibodies/*blood/immunology
MH  - Autoantigens/*immunology
MH  - Blotting, Western/methods
MH  - Case-Control Studies
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Humans
MH  - Hydrolases/blood/immunology
MH  - Immunoglobulin G/*immunology
MH  - *Protein Array Analysis
MH  - Protein Kinase C/blood/immunology
MH  - Protein Kinase C beta
MH  - Protein-Arginine Deiminase Type 4
MH  - Protein-Arginine Deiminases
MH  - Proto-Oncogene Proteins B-raf/blood/immunology
EDAT- 2008/10/30 09:00
MHDA- 2009/04/28 09:00
CRDT- 2008/10/30 09:00
PHST- 2008/10/30 09:00 [pubmed]
PHST- 2009/04/28 09:00 [medline]
PHST- 2008/10/30 09:00 [entrez]
AID - ard.2008.096917 [pii]
AID - 10.1136/ard.2008.096917 [doi]
PST - ppublish
SO  - Ann Rheum Dis. 2009 Apr;68(4):591-4. doi: 10.1136/ard.2008.096917. Epub 2008 Oct 
      28.