PMID- 18957789
OWN - NLM
STAT- MEDLINE
DCOM- 20090320
LR  - 20190819
IS  - 1346-9843 (Print)
IS  - 1346-9843 (Linking)
VI  - 72
IP  - 12
DP  - 2008 Dec
TI  - Transient increase of cytokines in the acute ischemic tissue is beneficial to 
      cell-based therapeutic angiogenesis.
PG  - 2075-80
AB  - BACKGROUND: Implantation of bone marrow cells (BMCs) is a treatment of ischemic 
      disease. It is well known that many inflammatory cytokines are released in 
      ischemic tissue, especially in the acute phase, so in the present study it was 
      investigated if the transient increase of cytokines in the acute ischemic tissue 
      influences cell-based therapeutic angiogenesis. METHODS AND RESULTS: Ischemic 
      limb models were created in C57BL/6 mice as 24 h (acute) or 2 weeks (chronic) 
      after ischemia. BMCs were cultured with total tissue protein, which was extracted 
      from the acute and chronic ischemic muscles. The survival, adhesion, and 
      migration of BMCs were significantly better after culture with 1 mg/ml total 
      tissue protein extracted from the acute ischemic limbs than from the chronic 
      ischemic limbs (p<0.001). For the in-vivo study, 8 x 10(6) BMCs, collected from 
      green fluorescent protein (GFP) transgenic mice, were implanted into the acute or 
      chronic ischemic limbs of the mice. The survival of implanted cells and blood 
      flow were significantly better when BMCs were implanted into the acute ischemic 
      limbs than into the chronic ischemic limbs (p<0.001). CONCLUSIONS: A transient 
      increase of cytokines in the acute ischemic tissue is beneficial for cell-based 
      therapeutic angiogenesis.
FAU - Qin, Shu-Lan
AU  - Qin SL
AD  - Department of Surgery and Clinical Science, Yamaguchi University, Graduate School 
      of Medicine, Ube, Japan.
FAU - Li, Tao-Sheng
AU  - Li TS
FAU - Kubo, Masayuki
AU  - Kubo M
FAU - Ohshima, Mako
AU  - Ohshima M
FAU - Furutani, Akira
AU  - Furutani A
FAU - Hamano, Kimikazu
AU  - Hamano K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081029
PL  - Japan
TA  - Circ J
JT  - Circulation journal : official journal of the Japanese Circulation Society
JID - 101137683
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Muscle Proteins)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - *Bone Marrow Transplantation
MH  - Cell Adhesion
MH  - Cell Movement
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Chronic Disease
MH  - Cytokines/*metabolism
MH  - Disease Models, Animal
MH  - Fibroblast Growth Factor 2/metabolism
MH  - Green Fluorescent Proteins/genetics
MH  - Hindlimb
MH  - Interleukin-1beta/metabolism
MH  - Ischemia/immunology/metabolism/physiopathology/*surgery
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Muscle Proteins/metabolism
MH  - Muscle, Skeletal/*blood supply/immunology/metabolism
MH  - *Neovascularization, Physiologic
MH  - Regional Blood Flow
MH  - Time Factors
MH  - Up-Regulation
EDAT- 2008/10/30 09:00
MHDA- 2009/03/21 09:00
CRDT- 2008/10/30 09:00
PHST- 2008/10/30 09:00 [pubmed]
PHST- 2009/03/21 09:00 [medline]
PHST- 2008/10/30 09:00 [entrez]
AID - JST.JSTAGE/circj/CJ-08-0392 [pii]
AID - 10.1253/circj.cj-08-0392 [doi]
PST - ppublish
SO  - Circ J. 2008 Dec;72(12):2075-80. doi: 10.1253/circj.cj-08-0392. Epub 2008 Oct 29.