PMID- 18958683
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20180131
IS  - 1547-6901 (Electronic)
IS  - 1547-691X (Linking)
VI  - 3
IP  - 1
DP  - 2006 Jan 1
TI  - A systematic analysis of the effect of corn, wheat, and poultry dusts on 
      interleukin-8 production by human respiratory epithelial cells.
PG  - 31-7
LID - 10.1080/15476910500496529 [doi]
AB  - The objective of this study was to determine the inflammatory response initiated 
      by various organic dusts by measuring in vitro interleukin-8 (I-8) production 
      from transformed respiratory epithelial cells after dust exposure. To accomplish 
      this objective, the following specific tasks were performed: 1) dust samples were 
      collected from grain farms, grain storage facilities, and poultry houses and 
      their endotoxin levels were determined; 2) A549 transformed respiratory 
      epithelial cells were exposed to 0, 10, 100, or 1,000 mug/ml of organic dust or 
      lipopolysaccharide (LPS); and, 3) production was measured using enzyme-linked 
      immunosorbant assays (ELISAs). The results indicate that LPS induced at all 
      concentrations. All dust types (corn, wheat, and poultry) induced at 
      significantly higher levels than control and at significantly different levels 
      between dust types. Overall there was an increasing trend of elevated production 
      as the concentration of dust exposure was increased except at the highest 
      concentration where IL-8 levels decreased and more cell death was observed. Corn 
      dust exposure induced IL-8 that increased with increasing dust concentration and 
      correlated with increasing amounts of LPS in the corn dust samples. However, IL-8 
      production in response to wheat dust was inversely related to LPS concentration 
      suggesting that other factors besides LPS are present in the dust that are 
      responsible for induction of IL-8 production. This study indicates that IL-8 
      induction varies between agricultural dust types and does not always correlate 
      with endotoxin levels present in the dust. Therefore, other factors may be 
      present in the dust that also elicit the immune response after exposure.
FAU - Redente, Elizabeth F
AU  - Redente EF
AD  - Department of Biology, Baylor University, Waco, Texas, USA.
FAU - Massengale, Rene D
AU  - Massengale RD
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Immunotoxicol
JT  - Journal of immunotoxicology
JID - 101201960
EDAT- 2008/10/30 09:00
MHDA- 2008/10/30 09:01
CRDT- 2008/10/30 09:00
PHST- 2008/10/30 09:00 [pubmed]
PHST- 2008/10/30 09:01 [medline]
PHST- 2008/10/30 09:00 [entrez]
AID - 742085018 [pii]
AID - 10.1080/15476910500496529 [doi]
PST - ppublish
SO  - J Immunotoxicol. 2006 Jan 1;3(1):31-7. doi: 10.1080/15476910500496529.