PMID- 18958741
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20081029
IS  - 1547-6901 (Electronic)
IS  - 1547-691X (Linking)
VI  - 4
IP  - 4
DP  - 2007 Oct
TI  - Identification of phenolic dermal sensitizers in a wound closure tape.
PG  - 303-10
LID - 10.1080/15476910701680236 [doi]
AB  - A latex-allergic patient presented with a severe local reaction to a non-latex 
      wound closure bandage following surgery. Extracts of the bandage were analyzed by 
      gas chromatograph-electron impact-mass spectrometry (GC EI-MS) in the total ion 
      monitoring mode. Components were identified by their ion mass fingerprint and 
      elution time as a corresponding standard from the GC column. The chemicals 
      identified were 4,4'-thiobis-(6-tert-butyl-m-cresol) (TBBC), 
      6-tert-Butyl-m-cresol (BC), 2,4-di-tert-butylphenol (BP) and erucamide (EA). 
      Sensitization potential of these chemicals was evaluated using two quantitative 
      structure-activity relationship (QSAR) programs. The phenol 
      2,6-di-tert-butyl-4-(hydroxymethyl)phenol (BHP) was also included in the test 
      series. It was initially thought to be present in the bandage but detectable 
      levels could not be confirmed. The potential for TBBC to induce a sensitization 
      response was predicted by both Derek for Windows and TOPKAT 6.2. The potential 
      for BC and BP to induce a sensitization response was predicted by Derek for 
      Windows, but not TOPKAT. BHP and EA were not predicted to be sensitizers by 
      either QSAR program. Local lymph node assay (LLNA) analysis of the chemicals 
      identified TBBC, BP, and BC as potential sensitizers with EC3 values between 0.2 
      and 4.5%. None of the animals exhibited body weight loss or skin irritation at 
      the concentrations tested. In agreement with the toxicological modeling, BHP did 
      not induce a sensitization response in the LLNA. Following a positive LLNA 
      response, TBBC, BP, and BC were further characterized by phenotypic analysis of 
      the draining lymph nodes. A positive LLNA result coupled with a lack of increase 
      in B220(+)IgE(+) cell and serum IgE characterize these chemicals as Type IV 
      sensitizers. These studies used a multidisciplinary approach combining clinical 
      observation, GC-EI-MS for chemical identification, QSAR modeling of chemicals 
      prior to animal testing, and the LLNA for determination of the sensitization 
      potential of chemicals in a manufactured product.
FAU - Myers, L P
AU  - Myers LP
AD  - National Institute for Occupational Safety and Health, Morgantown, West Virginia, 
      USA.
FAU - Law, B F
AU  - Law BF
FAU - Fedorowicz, A
AU  - Fedorowicz A
FAU - Siegel, P D
AU  - Siegel PD
FAU - Butterworth, L F
AU  - Butterworth LF
FAU - Anderson, S E
AU  - Anderson SE
FAU - Sussman, G
AU  - Sussman G
FAU - Shapiro, M
AU  - Shapiro M
FAU - Meade, B J
AU  - Meade BJ
FAU - Beezhold, D
AU  - Beezhold D
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Immunotoxicol
JT  - Journal of immunotoxicology
JID - 101201960
EDAT- 2008/10/30 09:00
MHDA- 2008/10/30 09:01
CRDT- 2008/10/30 09:00
PHST- 2008/10/30 09:00 [pubmed]
PHST- 2008/10/30 09:01 [medline]
PHST- 2008/10/30 09:00 [entrez]
AID - 787700265 [pii]
AID - 10.1080/15476910701680236 [doi]
PST - ppublish
SO  - J Immunotoxicol. 2007 Oct;4(4):303-10. doi: 10.1080/15476910701680236.