PMID- 18968024
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20190729
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 52
IP  - 4
DP  - 2000 Jul 31
TI  - Enzyme-octadecylamine Langmuir-Blodgett membranes for ENFET biosensors.
PG  - 663-70
AB  - Langmuir-Blodgett (LB) films containing butyrylcholinesterase (BuChE) are 
      fabricated to realise an enzymatic field effect transistor (ENFET) for the 
      detection of organophosphorus pesticides in water. Trichlorfon as a common 
      pesticide is examined in our work. The BuChE-immobilised LB films are formed by 
      adsorbing the enzyme molecules onto a stearylamine monolayer using the 
      electrostatic force. Enzyme/stearylamine mixed LB films are immobilized onto a 
      pH-ISFET surface and treated by glutaraldehyde vapour to improve the LB film's 
      stability. The ENFET thus obtained worked as a potentiometric biosensor for 
      trichlorfon detection on the basis of enzyme inhibition. The detection limit for 
      trichlorfon can reach 10(-7) M (26 ppb). The surface characteristics of 
      BuChE/stearylamine LB films obtained under various conditions of the dipping 
      surface pressures are analysed qualitatively by atomic force microscopy (AFM) and 
      analysed quantitatively by FTIR spectroscopy.
FAU - Wan, K
AU  - Wan K
AD  - IFOS, UMR CNRS 5621, Laboratory d'Ingenierie et de Fonctionnalisation des 
      Surfaces, Ecole Centrale de Lyon, BP 163, 69131 Ecully Cedex, France.
FAU - Chovelon, J M
AU  - Chovelon JM
FAU - Jaffrezic-Renault, N
AU  - Jaffrezic-Renault N
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
EDAT- 2008/10/31 09:00
MHDA- 2008/10/31 09:01
CRDT- 2008/10/31 09:00
PHST- 1999/12/13 00:00 [received]
PHST- 2000/03/23 00:00 [revised]
PHST- 2000/04/03 00:00 [accepted]
PHST- 2008/10/31 09:00 [pubmed]
PHST- 2008/10/31 09:01 [medline]
PHST- 2008/10/31 09:00 [entrez]
AID - S0039-9140(00)00407-0 [pii]
AID - 10.1016/s0039-9140(00)00407-0 [doi]
PST - ppublish
SO  - Talanta. 2000 Jul 31;52(4):663-70. doi: 10.1016/s0039-9140(00)00407-0.