PMID- 18968446
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130206
LR  - 20190729
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 55
IP  - 5
DP  - 2001 Dec 13
TI  - Whole-cell biodetection of halogenated organic acids.
PG  - 959-64
AB  - A whole-cell bacterial sensor system for short-chain halo-organic acids was 
      constructed, using 2-chloropropionic acid (2-CPA) as a model pollutant. An 
      Escherichia coli host was transformed with a moderate-copy plasmid containing a 
      fusion of two foreign genetic elements: (a) a promoter-containing segment of the 
      Pseudomonas DL-DEX (DL-2-haloacid dehalogenase) encoding gene and (b) 
      bioluminescence (luxCDABE) genes of Photorhabdus luminescens. The resulting 
      construct, named MT1, responded to the presence of 2-CPA by dose-dependent light 
      emission, in a highly specific albeit a very insensitive manner. Thus, while the 
      desired concept was successfully demonstrated, further genetic work is needed in 
      order to make such a construct practical for environmental monitoring purposes.
FAU - Tauber, M
AU  - Tauber M
AD  - Environmental Sciences, the Fredy and Nadine Herrmann Graduate School of Applied 
      Science, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
FAU - Rosen, R
AU  - Rosen R
FAU - Belkin, S
AU  - Belkin S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
EDAT- 2008/10/31 09:00
MHDA- 2008/10/31 09:01
CRDT- 2008/10/31 09:00
PHST- 2001/01/19 00:00 [received]
PHST- 2001/06/15 00:00 [revised]
PHST- 2001/06/21 00:00 [accepted]
PHST- 2008/10/31 09:00 [pubmed]
PHST- 2008/10/31 09:01 [medline]
PHST- 2008/10/31 09:00 [entrez]
AID - S0039-9140(01)00492-1 [pii]
AID - 10.1016/s0039-9140(01)00492-1 [doi]
PST - ppublish
SO  - Talanta. 2001 Dec 13;55(5):959-64. doi: 10.1016/s0039-9140(01)00492-1.