PMID- 18968760
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130211
LR  - 20190729
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 58
IP  - 2
DP  - 2002 Aug 23
TI  - Creatinine sensitive biosensor based on ISFETs and creatinine deiminase 
      immobilised in BSA membrane.
PG  - 351-7
AB  - A creatinine sensitive biosensor based on ion sensitive field-effect transistors 
      (ISFETs) with immobilised creatinine deiminase (CD) is developed. CD is 
      immobilised on the transducer surface by classical cross-linking with bovine 
      serum albumin (BSA) in a glutaraldehyde (GA) vapour. The linear dynamic ranges of 
      biosensors are between 0 and 5 mM creatinine concentration, and the sensor 
      sensitivity depends on the sample buffer concentration. Minimal detection limit 
      for creatinine determination in model solution with 144 mM NaCl and 5% BSA, pH 
      7.4, is about 10 muM. Biosensor responses are reproducible and stable during 
      continuous work at least for 8 h, and the relative standard deviation of sensor 
      response is approximately 3% (n=48, for creatinine concentrations of 0.2 and 0.6 
      mM). An investigation about storage stability of creatinine sensitive ENFETs kept 
      in dry at 4-6 degrees C shows that biosensors demonstrate an excellent storage 
      stability for at least 6 months and more. Moreover, creatinine sensitive 
      enzymatic field-effect transistors (ENFETs), demonstrating very good 
      performances, are very selective and specific and well suitable for hemodialysis 
      monitoring.
FAU - Soldatkin, Alexey P
AU  - Soldatkin AP
AD  - IFoS-UMR/CNRS 5621, Ecole Centrale de Lyon, 69131 36 Av. Guy de Collongue, F69134 
      Ecully, Cedex, France; Institute of Molecular Biology and Genetics, National 
      Academy of Sciences of Ukraine, 150 Zabolotnogo St., Kiev 03143, Ukraine.
FAU - Montoriol, Jean
AU  - Montoriol J
FAU - Sant, William
AU  - Sant W
FAU - Martelet, Claude
AU  - Martelet C
FAU - Jaffrezic-Renault, Nicole
AU  - Jaffrezic-Renault N
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
EDAT- 2008/10/31 09:00
MHDA- 2008/10/31 09:01
CRDT- 2008/10/31 09:00
PHST- 2002/03/11 00:00 [received]
PHST- 2002/05/17 00:00 [revised]
PHST- 2002/05/21 00:00 [accepted]
PHST- 2008/10/31 09:00 [pubmed]
PHST- 2008/10/31 09:01 [medline]
PHST- 2008/10/31 09:00 [entrez]
AID - S0039-9140(02)00283-7 [pii]
AID - 10.1016/s0039-9140(02)00283-7 [doi]
PST - ppublish
SO  - Talanta. 2002 Aug 23;58(2):351-7. doi: 10.1016/s0039-9140(02)00283-7.