PMID- 18969031
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20081030
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 60
IP  - 1
DP  - 2003 May 28
TI  - Determination of 2,4-dichlorophenoxyacetic acid and its major transformation 
      product in soil samples by liquid chromatographic analysis.
PG  - 115-21
LID - 10.1016/S0039-9140(03)00113-9 [doi]
AB  - The 2,4-dichlorophenoxyacetic acid (2,4-D) is one of the most applied herbicides 
      around the world to control broad leave herbs in many crops. In this study, a 
      method was developed for simultaneous extraction and determination of 2,4-D and 
      its major transformation product, i.e., the 2,4-dichlorophenol (2,4-DCP), in soil 
      samples. The herbicide and its degradation product were extracted twice from soil 
      samples, after acidification, by dichloromethane on ultrasound system for 1 h. 
      Both extracts were combined and filtrated in qualitative filter paper and 
      Celite((R)). The total extract was concentrated in rotatory evaporator, dried 
      under N(2) and finally dissolved in 1 ml of methanol. High Performance Liquid 
      Chromatography with UV detection at 230 nm was used for analysis. Recoveries were 
      obtained from soil samples fortified at 0.1, 1.0, 2.0, 3.0 and 4.0 mgkg(-1) 
      levels and the results varied from 85 to 111% (for 2,4-D) and from 95 to 98% (for 
      2,4-DCP). For both compounds, the limits of quantification were 0.1 mgkg(-1), 
      which were the loss level at which the accuracy and the precision were studied. 
      Nevertheless, the limits of detection, calculated by considering the blank 
      standard deviation and the minimum concentration level, were 0.03 and 0.02 
      mgkg(-1), for 2,4-D and 2,4-DCP, respectively. This proposed method was applied 
      to soil samples of eucalyptus crops, which was previously treated with the 
      herbicide. Despite that, neither 2,4-D nor its degradation product were detected 
      30 days after the herbicide application.
FAU - de Amarante, O P Jr
AU  - de Amarante OP Jr
AD  - Nucleus of Pesticide Residue Analysis (NARP), Department of Technological 
      Chemistry, Federal University of Maranhao, UFMA 65.080-040, Sao Luis, Maranhao, 
      Brazil.
FAU - Brito, N M
AU  - Brito NM
FAU - Dos Santos, T C R
AU  - Dos Santos TC
FAU - Nunes, G S
AU  - Nunes GS
FAU - Ribeiro, M L
AU  - Ribeiro ML
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
EDAT- 2008/10/31 09:00
MHDA- 2008/10/31 09:01
CRDT- 2008/10/31 09:00
PHST- 2002/07/24 00:00 [received]
PHST- 2003/01/14 00:00 [revised]
PHST- 2003/01/17 00:00 [accepted]
PHST- 2008/10/31 09:00 [pubmed]
PHST- 2008/10/31 09:01 [medline]
PHST- 2008/10/31 09:00 [entrez]
AID - S0039-9140(03)00113-9 [pii]
AID - 10.1016/S0039-9140(03)00113-9 [doi]
PST - ppublish
SO  - Talanta. 2003 May 28;60(1):115-21. doi: 10.1016/S0039-9140(03)00113-9.