PMID- 18969266
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130704
LR  - 20081030
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 62
IP  - 1
DP  - 2004 Jan 9
TI  - A rapid spectrophotometric method for the determination of transparent exopolymer 
      particles (TEP) in freshwater.
PG  - 81-5
LID - 10.1016/S0039-9140(03)00417-X [doi]
AB  - A simple and rapid spectrophotometric method is proposed for the determination of 
      transparent exopolymer particles (TEP) in freshwater samples. In this method, TEP 
      reacts with excess of alcian blue solution yielding a low solubility dye-TEP 
      complex. After centrifugation, the concentration of the remaining dye in the 
      supernatant was determined at 602nm and its concentration was related to the 
      concentration of TEP in freshwater. The effect of alcian blue concentration from 
      1.5x10(-3) to 9.0x10(-3)% (m/v), solution pH from 2.5 to 6.9 and stirring time 
      from 20 to 120s on the analytical curve was investigated. Under the optimum 
      conditions established, such as alcian blue concentration of 3.0x10(-3)% (m/v); 
      pH of 4.0 (0.2moll(-1) acetate buffer solution) and stirring time of 1min, the 
      analytical curve was linear from 0.50 to 10mugml(-1) (A=0.34-0.037[GX]; 
      r(2)=0.9999; where A is the absorbance and [GX] the gum xanthan concentration in 
      mugml(-1)) with a detection limit of 0.10mugml(-1). The recovery of TEP (as gum 
      xanthan) for two samples ranged from 95.3 to 108 and the relative standard 
      deviations (R.S.D.s) were lower than 0.8% for gum xanthan solutions at 
      concentrations of 1.0 and 1.5mugml(-1) (n=8). The results obtained for TEP in 
      freshwater samples using the proposed spectrophotometric method and those 
      obtained using a literature method are in agreement at the 95% confidence level 
      and within an acceptable range of error.
FAU - Arruda Fatibello, Silvia H S
AU  - Arruda Fatibello SH
AD  - Laboratorio de Ficologia, Departamento de Botanica, Centro de Ciencias Biologicas 
      e da Saude, Universidade Federal de Sao Carlos, Via Washington Luiz, km 235, 
      Caixa Postal 676, 13560-970, Sao Carlos, SP, Brazil.
FAU - Henriques Vieira, Armando Augusto
AU  - Henriques Vieira AA
FAU - Fatibello-Filho, Orlando
AU  - Fatibello-Filho O
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
EDAT- 2008/10/31 09:00
MHDA- 2008/10/31 09:01
CRDT- 2008/10/31 09:00
PHST- 2003/01/13 00:00 [received]
PHST- 2003/06/25 00:00 [revised]
PHST- 2003/06/25 00:00 [accepted]
PHST- 2008/10/31 09:00 [pubmed]
PHST- 2008/10/31 09:01 [medline]
PHST- 2008/10/31 09:00 [entrez]
AID - S0039-9140(03)00417-X [pii]
AID - 10.1016/S0039-9140(03)00417-X [doi]
PST - ppublish
SO  - Talanta. 2004 Jan 9;62(1):81-5. doi: 10.1016/S0039-9140(03)00417-X.