PMID- 18971252
OWN - NLM
STAT- MEDLINE
DCOM- 20081231
LR  - 20181113
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 36
IP  - 21
DP  - 2008 Dec
TI  - The antiretroviral potency of APOBEC1 deaminase from small animal species.
PG  - 6859-71
LID - 10.1093/nar/gkn802 [doi]
AB  - Although the role of the APOBEC3-dependent retroelement restriction system as an 
      intrinsic immune defense against human immunodeficiency virus type1 (HIV-1) 
      infection is becoming clear, only the rat ortholog of mammalian APOBEC1s (A1) 
      thus far has been shown to possess antiviral activity. Here, we cloned A1 cDNAs 
      from small animal species, and showed that similar to rat A1, both wild-type and 
      Deltavif HIV-1 infection was inhibited by mouse and hamster A1 (4- to 10-fold), 
      whereas human A1 had negligible effects. Moreover, rabbit A1 significantly 
      reduced the infectivity of both HIV-1 virions (>300-fold), as well as that of 
      SIVmac, SIVagm, FIV and murine leukemia virus. Immunoblot analysis showed that 
      A1s were efficiently incorporated into the HIV-1 virion, and their packaging is 
      mediated through an interaction with the nucleocapsid Gag domain. Interestingly, 
      there was a clear accumulation of particular C-T changes in the genomic RNAs of 
      HIV-1 produced in their presence, with few G-A changes in the proviral DNA. 
      Together, these data reveal that A1 may function as a defense mechanism, 
      regulating retroelements in a wide range of mammalian species.
FAU - Ikeda, Terumasa
AU  - Ikeda T
AD  - Department of Retrovirology and Self-Defense, Faculty of Medical and 
      Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan.
FAU - Ohsugi, Takeo
AU  - Ohsugi T
FAU - Kimura, Tetsuya
AU  - Kimura T
FAU - Matsushita, Shuzo
AU  - Matsushita S
FAU - Maeda, Yosuke
AU  - Maeda Y
FAU - Harada, Shinji
AU  - Harada S
FAU - Koito, Atsushi
AU  - Koito A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081029
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
RN  - EC 3.5.4.36 (APOBEC-1 Deaminase)
RN  - EC 3.5.4.36 (APOBEC1 protein, human)
RN  - EC 3.5.4.36 (Apobec1 protein, mouse)
RN  - EC 3.5.4.36 (Apobec1 protein, rat)
RN  - EC 3.5.4.5 (Cytidine Deaminase)
SB  - IM
MH  - APOBEC-1 Deaminase
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Cytidine Deaminase/classification/genetics/*metabolism
MH  - DNA, Viral/chemistry/metabolism
MH  - HIV-1/genetics/metabolism/*physiology
MH  - Humans
MH  - Lentivirus/*physiology
MH  - Leukemia Virus, Murine/physiology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Protein Structure, Tertiary
MH  - RNA, Viral/chemistry/metabolism
MH  - Rabbits
MH  - Rats
MH  - Virion/physiology
MH  - Virus Assembly
MH  - gag Gene Products, Human Immunodeficiency Virus/chemistry
PMC - PMC2588513
EDAT- 2008/10/31 09:00
MHDA- 2009/01/01 09:00
PMCR- 2008/10/29
CRDT- 2008/10/31 09:00
PHST- 2008/10/31 09:00 [pubmed]
PHST- 2009/01/01 09:00 [medline]
PHST- 2008/10/31 09:00 [entrez]
PHST- 2008/10/29 00:00 [pmc-release]
AID - gkn802 [pii]
AID - 10.1093/nar/gkn802 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2008 Dec;36(21):6859-71. doi: 10.1093/nar/gkn802. Epub 2008 
      Oct 29.