PMID- 18971368
OWN - NLM
STAT- MEDLINE
DCOM- 20090202
LR  - 20220408
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 47
IP  - 1
DP  - 2009 Jan
TI  - Phenotypic and genotypic analysis of enterotoxigenic Escherichia coli in samples 
      obtained from Egyptian children presenting to referral hospitals.
PG  - 189-97
LID - 10.1128/JCM.01282-08 [doi]
AB  - Hospital surveillance was established in the Nile River Delta to increase the 
      understanding of the epidemiology of diarrheal disease among Egyptian children. 
      Between September 2000 and August 2003, samples obtained from children less than 
      5 years of age who had diarrhea and who were seeking hospital care were cultured 
      for enteric bacteria. Colonies from each culture with a morphology typical of 
      that of Escherichia coli were tested for the heat-labile (LT) and heat-stable 
      (ST) toxins by a GM-1-specific enzyme-linked immunosorbent assay and colonization 
      factor (CF) antigens by an immunodot blot assay. Enterotoxigenic E. coli (ETEC) 
      isolates were recovered from 320/1,540 (20.7%) children, and ETEC isolates 
      expressing a known CF were identified in 151/320 (47%) samples. ST CFA/I, ST CS6, 
      ST CS14, and LT and ST CS5 plus CS6 represented 75% of the CFs expressed by ETEC 
      isolates expressing a detectable CF. Year-to-year variability in the proportion 
      of ETEC isolates that expressed a detectable CF was observed (e.g., the 
      proportion that expressed CFA/I ranged from 10% in year 1 to 21% in year 3); 
      however, the relative proportions of ETEC isolates expressing a CF were similar 
      over the reporting period. The proportion of CF-positive ETEC isolates was higher 
      among isolates that expressed ST. ETEC isolates expressing CS6 were isolated 
      significantly less often (P < 0.001) than isolates expressing CFA/I in children 
      less than 1 year of age. Macrorestriction profiling of CFA/I-expressing ETEC 
      isolates by using the restriction enzyme XbaI and pulsed-field gel 
      electrophoresis demonstrated a wide genetic diversity among the isolates that did 
      not directly correlate with the virulence of the pathogen. The genome plasticity 
      demonstrated in the ETEC isolates collected in this work suggests an additional 
      challenge to the development of a globally effective vaccine for ETEC.
FAU - Shaheen, H I
AU  - Shaheen HI
AD  - U.S. Naval Medical Research Unit No. 3, Cairo, Egypt. 
      hind.shaheen.eg@med.navy.mil
FAU - Abdel Messih, I A
AU  - Abdel Messih IA
FAU - Klena, J D
AU  - Klena JD
FAU - Mansour, A
AU  - Mansour A
FAU - El-Wakkeel, Z
AU  - El-Wakkeel Z
FAU - Wierzba, T F
AU  - Wierzba TF
FAU - Sanders, J W
AU  - Sanders JW
FAU - Khalil, S B
AU  - Khalil SB
FAU - Rockabrand, D M
AU  - Rockabrand DM
FAU - Monteville, M R
AU  - Monteville MR
FAU - Rozmajzl, P J
AU  - Rozmajzl PJ
FAU - Svennerholm, A M
AU  - Svennerholm AM
FAU - Frenck, R W
AU  - Frenck RW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081029
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Bacterial Toxins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (colonization factor antigens)
RN  - 0 (heat stable toxin (E coli))
RN  - 147680-16-8 (Fimbriae Proteins)
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
RN  - EC 3.1.21.- (endodeoxyribonuclease XBAI)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
SB  - IM
MH  - Bacterial Toxins/biosynthesis
MH  - Child, Preschool
MH  - Cluster Analysis
MH  - DNA Fingerprinting
MH  - DNA, Bacterial/metabolism
MH  - Deoxyribonucleases, Type II Site-Specific/metabolism
MH  - Diarrhea/epidemiology/*microbiology
MH  - Egypt/epidemiology
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Enterotoxigenic Escherichia coli/classification/*genetics/isolation & 
      purification/*metabolism
MH  - Enterotoxins/biosynthesis
MH  - Escherichia coli Infections/epidemiology/*microbiology
MH  - Escherichia coli Proteins/biosynthesis
MH  - Fimbriae Proteins/biosynthesis
MH  - Genetic Variation
MH  - Hospitals
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Molecular Epidemiology
MH  - Polymorphism, Restriction Fragment Length
PMC - PMC2620865
EDAT- 2008/10/31 09:00
MHDA- 2009/02/03 09:00
PMCR- 2009/07/01
CRDT- 2008/10/31 09:00
PHST- 2008/10/31 09:00 [pubmed]
PHST- 2009/02/03 09:00 [medline]
PHST- 2008/10/31 09:00 [entrez]
PHST- 2009/07/01 00:00 [pmc-release]
AID - JCM.01282-08 [pii]
AID - 1282-08 [pii]
AID - 10.1128/JCM.01282-08 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2009 Jan;47(1):189-97. doi: 10.1128/JCM.01282-08. Epub 2008 Oct 
      29.