PMID- 18973100
OWN - NLM
STAT- MEDLINE
DCOM- 20090616
LR  - 20130731
IS  - 1018-9068 (Print)
IS  - 1018-9068 (Linking)
VI  - 18
IP  - 5
DP  - 2008
TI  - Allergen-sensitization increases mast-cell expression of the exocytotic proteins 
      SNAP-23 and syntaxin 4, which are involved in histamine secretion.
PG  - 366-71
AB  - BACKGROUND: Activation of mast cells (MCs) via aggregation of immunoglobulin E 
      (IgE) bound to its high affinity receptor (FcepsilonRI) results in release of 
      inflammatory mediators from secretory granules. Histamine is one of the critical 
      biological mediators released in the allergic response. Synaptosomal-associated 
      protein of 23 kDa (SNAP-23) and syntaxin 4 are plasma membrane proteins that have 
      been associated with exocytosis in MCs. Studies with monoclonal IgEs indicate 
      that binding of IgE to FcepsilonRI induces molecular and biological changes in 
      OBJECTIVES: The aim of this study was to investigate changes in the expression of 
      SNAP-23 and syntaxin 4 by MCs following rat sensitization with ovalbumin (OVA). 
      In addition, we assessed whether these proteins were involved in histamine 
      secretion. METHODS: SNAP-23 and syntaxin 4 expression was analyzed by Western 
      blot using MCs from control and sensitized animals. Successful sensitization was 
      confirmed based on the passive cutaneous anaphylaxis reaction. To test the role 
      of these exocytotic proteins in histamine secretion, permeabilized MCs were 
      incubated with SNAP-23 and syntaxin 4 antibodies. RESULTS: Expression of SNAP-23 
      and syntaxin 4 was significantly higher in MCs from OVA-sensitized rats than in 
      cells from control animals. In addition, incubation of permabilized cells with 
      antibodies to SNAP-23 and syntaxin 4 led to a marked reduction of histamine 
      secretion in stimulated cells. CONCLUSIONS: Sensitization with OVA increases the 
      expression of SNAP-23 and syntaxin 4 in MCs. Furthermore, our data suggest that 
      these exocytotic proteins participate in histamine secretion.
FAU - Salinas, E
AU  - Salinas E
AD  - Department of Microbiology, Basic Science Center, Autonomous University of 
      Aguascalientes, Mexico. emsalin@correo.uaa.mx
FAU - Quintanar-Stephano, A
AU  - Quintanar-Stephano A
FAU - Cordova, L E
AU  - Cordova LE
FAU - Ouintanar, J L
AU  - Ouintanar JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Spain
TA  - J Investig Allergol Clin Immunol
JT  - Journal of investigational allergology & clinical immunology
JID - 9107858
RN  - 0 (Qa-SNARE Proteins)
RN  - 0 (Qb-SNARE Proteins)
RN  - 0 (Qc-SNARE Proteins)
RN  - 0 (SNAP23 protein, human)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Capillary Permeability/genetics/immunology
MH  - Cells, Cultured
MH  - *Desensitization, Immunologic
MH  - Exocytosis/genetics/*immunology
MH  - Gene Expression Regulation
MH  - Histamine Release/genetics/*immunology
MH  - Immunization
MH  - Male
MH  - Mast Cells/immunology/*metabolism/pathology
MH  - Ovalbumin/administration & dosage
MH  - Passive Cutaneous Anaphylaxis/genetics/immunology
MH  - Qa-SNARE Proteins/genetics/*immunology/*metabolism
MH  - Qb-SNARE Proteins/genetics/*immunology/*metabolism
MH  - Qc-SNARE Proteins/genetics/*immunology/*metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2008/11/01 09:00
MHDA- 2009/06/17 09:00
CRDT- 2008/11/01 09:00
PHST- 2008/11/01 09:00 [pubmed]
PHST- 2009/06/17 09:00 [medline]
PHST- 2008/11/01 09:00 [entrez]
PST - ppublish
SO  - J Investig Allergol Clin Immunol. 2008;18(5):366-71.