PMID- 18973528
OWN - NLM
STAT- MEDLINE
DCOM- 20091029
LR  - 20171116
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 44
IP  - 4
DP  - 2009 Aug
TI  - Porphyromonas gingivalis fimbriae induce unique dendritic cell subsets via 
      Toll-like receptor 2.
PG  - 543-9
LID - 10.1111/j.1600-0765.2008.01149.x [doi]
AB  - BACKGROUND AND OBJECTIVE: Dendritic cells (DCs) play a critical role in the 
      activation of T cells as well as in shaping immune responses. We have reported 
      previously that Porphyromonas gingivalis lipopolysaccharides (Pg LPS) induced a 
      CD14(+)CD16(+) DC subset with a weak immuno-stimulatory activity. In contrast, 
      Escherichia coli LPS (Ec LPS) induced fully matured DCs with strong 
      immunostimulatory activities. Since Pg LPS as well as Pg fimbriae have been 
      indicated to work as Toll-like receptor (TLR) 2 ligands, we speculate that the 
      TLR usage of bacterial antigens may be critical for DC maturation. MATERIAL AND 
      METHODS: We investigated the effect of Pg fimbriae on the phenotype and function 
      of human peripheral blood DCs in comparison with a TLR2 ligand, peptidoglycan, 
      and a TLR4 ligand, Ec LPS. RESULTS: Flow cytometry revealed that Pg fimbriae and 
      peptidoglycan but not Ec LPS induced CD14 and CD16 expression on peripheral blood 
      DCs (CD14(-)CD16(-)). A monoclonal antibody against TLR2 abrogated this 
      induction, but an antibody against TLR4 had no effect. Dendritic cells stimulated 
      with Pg fimbriae had a weaker capability to induce allogenic T cell proliferation 
      and exhibited a weaker production of interleukin-8 and regulated upon activation, 
      normal T cell expressed and secreted (RANTES) than DCs stimulated with Ec LPS. 
      CONCLUSION: These results indicate that different TLR usage affects mature DC 
      phenotype and function and is thus crucial to the regulation of immunity to the 
      pathogen.
FAU - Kanaya, S
AU  - Kanaya S
AD  - Division of Periodontology and Endodontology, Tohoku University Graduate School 
      of Dentistry, Sendai, Japan.
FAU - Nemoto, E
AU  - Nemoto E
FAU - Ogawa, T
AU  - Ogawa T
FAU - Shimauchi, H
AU  - Shimauchi H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091007
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (CCL5 protein, human)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Peptidoglycan)
RN  - 0 (Receptors, IgG)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Cell Proliferation
MH  - Chemokine CCL5/immunology
MH  - Dendritic Cells/*immunology
MH  - Escherichia coli/immunology
MH  - Fimbriae, Bacterial/*immunology
MH  - Flow Cytometry
MH  - Humans
MH  - Interleukin-8/immunology
MH  - Leukocytes, Mononuclear/immunology
MH  - Lipopolysaccharide Receptors/immunology
MH  - Lipopolysaccharides/immunology
MH  - Lymphocyte Activation/immunology
MH  - Peptidoglycan/immunology
MH  - Phenotype
MH  - Porphyromonas gingivalis/*immunology
MH  - Receptors, IgG/immunology
MH  - T-Lymphocytes/immunology
MH  - Toll-Like Receptor 2/*immunology
MH  - Toll-Like Receptor 4/immunology
EDAT- 2008/11/01 09:00
MHDA- 2009/10/30 06:00
CRDT- 2008/11/01 09:00
PHST- 2008/11/01 09:00 [pubmed]
PHST- 2009/10/30 06:00 [medline]
PHST- 2008/11/01 09:00 [entrez]
AID - JRE1149 [pii]
AID - 10.1111/j.1600-0765.2008.01149.x [doi]
PST - ppublish
SO  - J Periodontal Res. 2009 Aug;44(4):543-9. doi: 10.1111/j.1600-0765.2008.01149.x. 
      Epub 2009 Oct 7.