PMID- 18973625
OWN - NLM
STAT- MEDLINE
DCOM- 20081217
LR  - 20131121
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 10
IP  - 11
DP  - 2008 Nov
TI  - Environmental detection of octahaem cytochrome c hydroxylamine/hydrazine 
      oxidoreductase genes of aerobic and anaerobic ammonium-oxidizing bacteria.
PG  - 3140-9
LID - 10.1111/j.1462-2920.2008.01732.x [doi]
AB  - Bacterial aerobic ammonium oxidation and anaerobic ammonium oxidation (anammox) 
      are important processes in the global nitrogen cycle. Key enzymes in both 
      processes are the octahaem cytochrome c (OCC) proteins, hydroxylamine 
      oxidoreductase (HAO) of aerobic ammonium-oxidizing bacteria (AOB), which 
      catalyses the oxidation of hydroxylamine to nitrite, and hydrazine oxidoreductase 
      (HZO) of anammox bacteria, which converts hydrazine to N(2). While the genomes of 
      AOB encode up to three nearly identical copies of hao operons, genome analysis of 
      Candidatus'Kuenenia stuttgartiensis' showed eight highly divergent octahaem 
      protein coding regions as possible candidates for the HZO. Based on their 
      phylogenetic relationship and biochemical characteristics, the sequences of these 
      eight gene products grouped in three clusters. Degenerate primers were designed 
      on the basis of available gene sequences with the aim to detect hao and hzo genes 
      in various ecosystems. The hao primer pairs amplified gene fragments from 738 to 
      1172 bp and the hzo primer pairs amplified gene fragments from 289 to 876 bp in 
      length, when tested on genomic DNA isolated from a variety of AOB and anammox 
      bacteria. A selection of these primer pairs was also used successfully to amplify 
      and analyse the hao and hzo genes in community DNA isolated from different 
      ecosystems harbouring both AOB and anammox bacteria. We propose that OCC 
      protein-encoding genes are suitable targets for molecular ecological studies on 
      both aerobic and anaerobic ammonium-oxidizing bacteria.
FAU - Schmid, Markus C
AU  - Schmid MC
AD  - Department of Microbiology, IWWR, Radboud University Nijmegen, Nijmegen, the 
      Netherlands. mschmid@science.ru.nl
FAU - Hooper, Alan B
AU  - Hooper AB
FAU - Klotz, Martin G
AU  - Klotz MG
FAU - Woebken, Dagmar
AU  - Woebken D
FAU - Lam, Phyllis
AU  - Lam P
FAU - Kuypers, Marcel M M
AU  - Kuypers MM
FAU - Pommerening-Roeser, Andreas
AU  - Pommerening-Roeser A
FAU - Op den Camp, Huub J M
AU  - Op den Camp HJ
FAU - Jetten, Mike S M
AU  - Jetten MS
LA  - eng
SI  - GENBANK/FM163618
SI  - GENBANK/FM163619
SI  - GENBANK/FM163620
SI  - GENBANK/FM163621
SI  - GENBANK/FM163622
SI  - GENBANK/FM163623
SI  - GENBANK/FM163624
SI  - GENBANK/FM163625
SI  - GENBANK/FM163626
SI  - GENBANK/FM163627
SI  - GENBANK/FM163628
SI  - GENBANK/FM163629
SI  - GENBANK/FM163630
SI  - GENBANK/FM163631
SI  - GENBANK/FM163632
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Bacterial Proteins)
RN  - 0 (Cytochrome c Group)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Hydrazines)
RN  - 27RFH0GB4R (hydrazine)
RN  - 2FP81O2L9Z (Hydroxylamine)
RN  - 9035-44-3 (cytochrome c(3))
RN  - EC 1.- (Oxidoreductases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Proteins/*genetics
MH  - Cytochrome c Group/*metabolism
MH  - DNA Primers
MH  - DNA, Bacterial/chemistry/genetics
MH  - *Environmental Microbiology
MH  - Hydrazines/*metabolism
MH  - Hydroxylamine/*metabolism
MH  - Molecular Sequence Data
MH  - Oxidoreductases/*genetics
MH  - Phylogeny
MH  - Polymerase Chain Reaction/*methods
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 2008/11/01 09:00
MHDA- 2008/12/18 09:00
CRDT- 2008/11/01 09:00
PHST- 2008/11/01 09:00 [pubmed]
PHST- 2008/12/18 09:00 [medline]
PHST- 2008/11/01 09:00 [entrez]
AID - EMI1732 [pii]
AID - 10.1111/j.1462-2920.2008.01732.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2008 Nov;10(11):3140-9. doi: 10.1111/j.1462-2920.2008.01732.x.