PMID- 18975342
OWN - NLM
STAT- MEDLINE
DCOM- 20090107
LR  - 20221207
IS  - 0004-3591 (Print)
IS  - 0004-3591 (Linking)
VI  - 58
IP  - 11
DP  - 2008 Nov
TI  - A potential role of thymic stromal lymphopoietin in the recruitment of 
      macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 
      induction: implications for herniated discs.
PG  - 3510-9
LID - 10.1002/art.23965 [doi]
AB  - OBJECTIVE: To determine whether thymic stromal lymphopoietin (TSLP) plays a role 
      in the resorption of herniated disc tissue. METHODS: The expression of TSLP 
      messenger RNA (mRNA) and protein in mouse intervertebral disc cells was assessed 
      by quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent 
      assay (ELISA), and immunohistochemical analysis. The ability of mouse 
      intervertebral disc cells to respond to TSLP stimulation was examined by Western 
      blot analysis, ELISA, and protein array analysis. Intracellular signaling 
      pathways involved in TSLP signaling in mouse intervertebral disc cells were 
      investigated using several chemical inhibitors. The role of TSLP in macrophage 
      migration into the intervertebral disc was assessed by in vitro migration assay. 
      Finally, TSLP expression in clinical specimens derived from patients with a 
      herniated disc was examined by immunohistochemistry. RESULTS: Mouse 
      intervertebral disc cells expressed TSLP mRNA and protein upon stimulation with 
      NF-kappaB-activating ligands such as tumor necrosis factor alpha. In addition, 
      the mouse intervertebral disc cells expressed the TSLP receptor and produced 
      monocyte chemotactic protein 1 (MCP-1; CCL2) and macrophage colony-stimulating 
      factor in response to TSLP stimulation. Both anulus fibrosus and nucleus pulposus 
      intervertebral disc cells expressed MCP-1 upon TSLP stimulation, which was 
      mediated via the phosphatidylinositol 3-kinase/Akt pathway. Consistently, the 
      supernatants of TSLP-activated intervertebral disc cultures had the capacity to 
      induce macrophage migration in an MCP-1-dependent manner. Finally, TSLP and MCP-1 
      were coexpressed in human herniated disc specimens in which macrophage 
      infiltration into the tissue was observed. CONCLUSION: TSLP induced by 
      NF-kappaB-activating ligands in intervertebral discs may contribute to the 
      recruitment of macrophages to the intervertebral disc by stimulating MCP-1 
      production and may be involved in the resorption of herniated disc tissue.
FAU - Ohba, Tetsuro
AU  - Ohba T
AD  - University of Yamanashi, Yamanashi, Japan.
FAU - Haro, Hirotaka
AU  - Haro H
FAU - Ando, Takashi
AU  - Ando T
FAU - Koyama, Kensuke
AU  - Koyama K
FAU - Hatsushika, Kyosuke
AU  - Hatsushika K
FAU - Suenaga, Fumiko
AU  - Suenaga F
FAU - Ohnuma, Yuko
AU  - Ohnuma Y
FAU - Nakamura, Yuki
AU  - Nakamura Y
FAU - Katoh, Ryohei
AU  - Katoh R
FAU - Ogawa, Hideoki
AU  - Ogawa H
FAU - Hamada, Yoshiki
AU  - Hamada Y
FAU - Nakao, Atsuhito
AU  - Nakao A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arthritis Rheum
JT  - Arthritis and rheumatism
JID - 0370605
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - GT0IL38SP4 (Thymic Stromal Lymphopoietin)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Bone Resorption/*physiopathology
MH  - Cell Movement/physiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Cytokines/*physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunohistochemistry
MH  - Intervertebral Disc/*cytology/physiology
MH  - Intervertebral Disc Displacement/*physiopathology
MH  - Macrophages/*physiology
MH  - Mice
MH  - NF-kappa B/physiology
MH  - Proteins/analysis
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Thymic Stromal Lymphopoietin
EDAT- 2008/11/01 09:00
MHDA- 2009/01/08 09:00
CRDT- 2008/11/01 09:00
PHST- 2008/11/01 09:00 [pubmed]
PHST- 2009/01/08 09:00 [medline]
PHST- 2008/11/01 09:00 [entrez]
AID - 10.1002/art.23965 [doi]
PST - ppublish
SO  - Arthritis Rheum. 2008 Nov;58(11):3510-9. doi: 10.1002/art.23965.