PMID- 18977270
OWN - NLM
STAT- MEDLINE
DCOM- 20090311
LR  - 20081202
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 27
IP  - 1
DP  - 2009 Jan 1
TI  - Vaccinia virus proteins activate human dendritic cells to induce T cell responses 
      in vitro.
PG  - 88-92
LID - 10.1016/j.vaccine.2008.10.031 [doi]
AB  - The mechanisms of protection elicited by smallpox vaccine in humans have not been 
      delineated. Dendritic cells (DCs) are the most potent of professional antigen 
      presenting cells which on activation modulate both innate and adaptive immune 
      responses. Immunogenic proteins of vaccinia virus have been identified with 
      protein microarray technology. We have investigated the effect of three most 
      immunogenic proteins, D8L, D13L, and H3L on human monocyte-derived DCs with a 
      view to understand the mechanisms underlying the immunogenicity of these 
      proteins. Our data show that all three proteins activate and mature DCs, induce 
      secretion of cytokines IL-12p70, IL-10, TNF-alpha, IL-6 from DCs, and rVV 
      protein-loaded DCs induce secretion of IFN-gamma and proliferation of T cells 
      with selective expansion of effector memory T cells. Overall D13L and H3L 
      proteins are more immunogenic than D8L. Taken together data suggest that these 
      peptides are highly immunogenic and can modulate an effective innate and adaptive 
      response in humans in vitro.
FAU - Agrawal, Sudhanshu
AU  - Agrawal S
AD  - Division of Basic and Clinical Immunology, University of California, Irvine, CA 
      92697, USA.
FAU - Gupta, Sudhir
AU  - Gupta S
FAU - Agrawal, Anshu
AU  - Agrawal A
LA  - eng
PT  - Journal Article
DEP - 20081031
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Cytokines)
RN  - 0 (Viral Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Cells, Cultured
MH  - Cytokines/immunology
MH  - Dendritic Cells/*drug effects/*immunology
MH  - Humans
MH  - Interferon-gamma/immunology
MH  - T-Lymphocytes/*immunology
MH  - Vaccinia virus/*immunology
MH  - Viral Proteins/immunology/*pharmacology
EDAT- 2008/11/04 09:00
MHDA- 2009/03/12 09:00
CRDT- 2008/11/04 09:00
PHST- 2008/08/18 00:00 [received]
PHST- 2008/09/25 00:00 [revised]
PHST- 2008/10/07 00:00 [accepted]
PHST- 2008/11/04 09:00 [pubmed]
PHST- 2009/03/12 09:00 [medline]
PHST- 2008/11/04 09:00 [entrez]
AID - S0264-410X(08)01402-3 [pii]
AID - 10.1016/j.vaccine.2008.10.031 [doi]
PST - ppublish
SO  - Vaccine. 2009 Jan 1;27(1):88-92. doi: 10.1016/j.vaccine.2008.10.031. Epub 2008 
      Oct 31.