PMID- 18977502
OWN - NLM
STAT- MEDLINE
DCOM- 20090116
LR  - 20200423
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 383
IP  - 1
DP  - 2009 Jan 5
TI  - Reverse genetic manipulation of the overlapping coding regions for structural 
      proteins of the type II porcine reproductive and respiratory syndrome virus.
PG  - 22-31
LID - 10.1016/j.virol.2008.09.013 [doi]
AB  - The overlapping genomic regions coding for structural proteins of porcine 
      reproductive and respiratory syndrome virus (PRRSV) poses problems for molecular 
      dissection of the virus replication process. We constructed five mutant 
      full-length cDNA clones with the overlapping regions unwound and 1 to 3 
      restriction sites inserted between two adjacent ORFs (ORF1/2, ORF4/5, ORF5/6, ORF 
      6/7 and ORF7/3' UTR), which generated the recombinant viruses. Our findings 
      demonstrated that 1) the overlapping structural protein ORFs can be physically 
      separated, and is dispensable for virus viability; 2) such ORF separations did 
      not interrupt the subgenomic RNA synthesis; 3) the plaque morphology, growth 
      kinetics, and antigenicity of these mutant viruses were virtually 
      indistinguishable from those of the parental virus in cultured cells; and 4) 
      these mutant viruses remained genetic stable in vitro. This study lays a 
      foundation for further molecular dissection of PRRSV replication process, and 
      development of genetically tagged vaccines against PRRS.
FAU - Yu, Dandan
AU  - Yu D
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, 
      Chinese Ministry of Agriculture, Shanghai 200241, China.
FAU - Lv, Jian
AU  - Lv J
FAU - Sun, Zhi
AU  - Sun Z
FAU - Zheng, Haihong
AU  - Zheng H
FAU - Lu, Jiaqi
AU  - Lu J
FAU - Yuan, Shishan
AU  - Yuan S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081105
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antigens, Viral)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Antigens, Viral/immunology
MH  - Genome, Viral
MH  - Microbial Viability
MH  - Models, Molecular
MH  - Molecular Biology/methods
MH  - Nucleic Acid Conformation
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/physiology
MH  - RNA, Viral/biosynthesis/genetics
MH  - Recombination, Genetic
MH  - Viral Plaque Assay
MH  - Viral Structural Proteins/*genetics
MH  - Virus Replication
PMC - PMC7172853
EDAT- 2008/11/04 09:00
MHDA- 2009/01/17 09:00
PMCR- 2008/11/05
CRDT- 2008/11/04 09:00
PHST- 2008/05/29 00:00 [received]
PHST- 2008/06/28 00:00 [revised]
PHST- 2008/09/07 00:00 [accepted]
PHST- 2008/11/04 09:00 [pubmed]
PHST- 2009/01/17 09:00 [medline]
PHST- 2008/11/04 09:00 [entrez]
PHST- 2008/11/05 00:00 [pmc-release]
AID - S0042-6822(08)00594-1 [pii]
AID - 10.1016/j.virol.2008.09.013 [doi]
PST - ppublish
SO  - Virology. 2009 Jan 5;383(1):22-31. doi: 10.1016/j.virol.2008.09.013. Epub 2008 
      Nov 5.