PMID- 18979063
OWN - NLM
STAT- MEDLINE
DCOM- 20090608
LR  - 20181201
IS  - 1591-8890 (Print)
IS  - 1591-8890 (Linking)
VI  - 9
IP  - 1
DP  - 2009 Mar
TI  - Cord blood dendritic cells prevent the differentiation of naive T-helper cells 
      towards Th1 irrespective of their subtype.
PG  - 29-36
LID - 10.1007/s10238-008-0020-2 [doi]
AB  - Allogeneic cord blood transplantation is associated with a less severe 
      graft-versus-host disease (GVHD). This observation is thought to be due to 
      immaturity of cord blood cell immune capabilities. Dendritic cells (DCs) are the 
      most potent antigen-presenting cells of the immune system capable of initiation 
      and regulation of immune responses. In this investigation, we hypothesized that 
      non-manipulated cord blood dendritic cells (CBDCs) not only differ in their 
      functional maturity from adult peripheral blood DCs (PBDCs) but also differ in 
      their subsets and their preference in promoting Th1 or Th2 immune responses. 
      Non-manipulated fresh DCs were isolated from cord blood (CB) and adult peripheral 
      blood (PB) mononuclear cells as lineage marker negative cells. The differences in 
      expression of costimulatory molecules, the proportion of myeloid and lymphoid DCs 
      subsets, their immunostimulatory characteristics and their influence on promoting 
      the differentiation of naive T cells towards Th1 or Th2 cells were then 
      investigated in these two populations. Our results showed that freshly isolated 
      CBDCs, similar to cord blood monocyte derived DCs, were poor inducers of 
      IFN-gamma secretion while they increased the induction of IL-4 production by T 
      cells in comparison with PBDCs. CBDCs were also poor stimulators of allogenic T 
      cells in mixed leukocyte reaction compared to adult peripheral blood dendritic 
      cells. They also displayed decreased expression of HLA-DR and CD86 molecules. The 
      ratio of lymphoid DCs (CD11c(-), CD123(+)) to myeloid DCs (CD11c(+), CD123(-)) 
      was significantly higher in CB compared to PB. We conclude that CBDCs 
      preferential priming of naive T cells towards Th2 population, seems to be an 
      intrinsic property independent of their subtype. This property along with their 
      functional immaturity should contribute to outcome of cord blood transplantation.
FAU - Naderi, Nadereh
AU  - Naderi N
AD  - Department of Immunology, Tarbiat Modares University, P.O. Box 14115-331, Tehran, 
      Iran.
FAU - Pourfathollah, Ali Akbar
AU  - Pourfathollah AA
FAU - Alimoghaddam, Kamran
AU  - Alimoghaddam K
FAU - Moazzeni, Seyed Mohammad
AU  - Moazzeni SM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081101
PL  - Italy
TA  - Clin Exp Med
JT  - Clinical and experimental medicine
JID - 100973405
RN  - 0 (IL4 protein, human)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cell Differentiation/*immunology
MH  - Cord Blood Stem Cell Transplantation
MH  - Dendritic Cells/*immunology
MH  - Fetal Blood/*cytology
MH  - Graft vs Host Disease/immunology
MH  - Humans
MH  - Infant, Newborn
MH  - Interferon-gamma/metabolism
MH  - Interleukin-4/biosynthesis
MH  - T-Lymphocytes, Helper-Inducer/*immunology
MH  - Th1 Cells/*immunology
MH  - Th2 Cells/*immunology
EDAT- 2008/11/04 09:00
MHDA- 2009/06/09 09:00
CRDT- 2008/11/04 09:00
PHST- 2008/02/24 00:00 [received]
PHST- 2008/09/03 00:00 [accepted]
PHST- 2008/11/04 09:00 [pubmed]
PHST- 2009/06/09 09:00 [medline]
PHST- 2008/11/04 09:00 [entrez]
AID - 10.1007/s10238-008-0020-2 [doi]
PST - ppublish
SO  - Clin Exp Med. 2009 Mar;9(1):29-36. doi: 10.1007/s10238-008-0020-2. Epub 2008 Nov 
      1.