PMID- 18980527
OWN - NLM
STAT- MEDLINE
DCOM- 20090212
LR  - 20081104
IS  - 0022-3492 (Print)
IS  - 0022-3492 (Linking)
VI  - 79
IP  - 11
DP  - 2008 Nov
TI  - Lipopolysaccharide-mediated enhancement of bone metabolism in estrogen-deficient 
      mice.
PG  - 2173-81
LID - 10.1902/jop.2008.070127 [doi]
AB  - BACKGROUND: Osteoporosis may be a risk factor in periodontal disease. However, 
      biologic mechanisms explaining the apparent interaction between these two 
      diseases have not been defined. It is well known that lipopolysaccharide (LPS) 
      increases the resorption of alveolar bone. We hypothesized that LPS and estrogen 
      deficiency have synergistic effects on bone metabolism and may lead to enhanced 
      bone resorption. METHODS: Eighty 8-week-old female ddY mice were divided into two 
      groups and underwent a sham operation or bilateral ovariectomy. They were 
      maintained for 4 weeks to assess estrogen-deficient bone loss. Osteoblasts and 
      bone marrow cells (BMCs) were collected from ovariectomized (OVX) and 
      sham-operated mice. Osteoclast differentiation in a coculture of osteoblasts and 
      BMCs was investigated by tartrate-resistant acid phosphatase staining. Receptor 
      activator of nuclear factor-kappa B ligand (RANKL) mRNA expression in LPS-treated 
      osteoblasts was investigated using real-time polymerase chain reaction. 
      Interferon-gamma (IFN-gamma) and interleukin (IL)-6 and -10 levels in the culture 
      supernatants were evaluated by enzyme-linked immunosorbent assay. Group means 
      were compared by analysis of variance followed by Tukey's honestly significant 
      difference. RESULTS: There was a significant increase in the number of 
      osteoclasts in LPS-treated cocultures from OVX mice compared to sham controls (P 
      <0.05). RANKL mRNA expression in LPS-treated osteoblasts from OVX mice was 
      greater than in sham mice (P <0.05). In contrast, the production of IFN-gamma in 
      LPS-treated coculture from OVX mice was significantly lower than in sham mice (P 
      <0.05). CONCLUSION: LPS-bearing Gram-negative organisms are abundant in 
      periodontal disease, and the results supported the hypothesis that bone 
      resorption is increased in estrogen-deficient patients.
FAU - Fujita, Sachiko
AU  - Fujita S
AD  - Department of Periodontology, Aichi-Gakuin University, Nagoya, Japan.
FAU - Kikuchi, Takeshi
AU  - Kikuchi T
FAU - Sobue, Takanori
AU  - Sobue T
FAU - Suzuki, Mariyo
AU  - Suzuki M
FAU - Koide, Masanori
AU  - Koide M
FAU - Noguchi, Toshihide
AU  - Noguchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Estrogens)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RANK Ligand)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/*metabolism
MH  - Animals
MH  - Bone Remodeling/immunology/*physiology
MH  - Bone Resorption/immunology/*metabolism
MH  - Bone and Bones/cytology/*metabolism
MH  - Estrogens/*deficiency/physiology
MH  - Female
MH  - Interleukin-1/metabolism
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides/*immunology/metabolism
MH  - Mice
MH  - Osteoclasts/immunology/physiology
MH  - Postmenopause/metabolism
MH  - RANK Ligand/genetics/metabolism
MH  - RNA, Messenger/analysis
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2008/11/05 09:00
MHDA- 2009/02/13 09:00
CRDT- 2008/11/05 09:00
PHST- 2008/11/05 09:00 [pubmed]
PHST- 2009/02/13 09:00 [medline]
PHST- 2008/11/05 09:00 [entrez]
AID - 10.1902/jop.2008.070127 [doi]
PST - ppublish
SO  - J Periodontol. 2008 Nov;79(11):2173-81. doi: 10.1902/jop.2008.070127.