PMID- 18980528
OWN - NLM
STAT- MEDLINE
DCOM- 20090212
LR  - 20161124
IS  - 0022-3492 (Print)
IS  - 0022-3492 (Linking)
VI  - 79
IP  - 11
DP  - 2008 Nov
TI  - Effect of neurotrophin-4/5 on bone/cementum-related protein expressions and DNA 
      synthesis in cultures of human periodontal ligament cells.
PG  - 2182-9
LID - 10.1902/jop.2008.070402 [doi]
AB  - BACKGROUND: We studied neurotrophins (NTs) as signaling molecules for periodontal 
      tissue regeneration and showed that nerve growth factor (NGF) and brain-derived 
      neurotrophic factor (BDNF) modulate the proliferation and differentiation of 
      human periodontal ligament (HPL) cells in vitro. The purpose of this study was to 
      investigate whether NT-4/5 also has the ability to regulate the function of HPL 
      cells. METHODS: mRNA expressions of NT-4/5 and its high-affinity tyrosine kinase 
      receptor (trk)B were analyzed in HPL cells by reverse transcription-polymerase 
      chain reaction. We examined how NT-4/5 regulates the mRNA expression of 
      bone/cementum-related proteins (alkaline phosphatase [ALPase], osteopontin [OPN], 
      osteocalcin [OC], and bone morphogenetic protein [BMP]-2) in cultures of HPL 
      cells. Moreover, the effects of NT-4/5 on calcification, the production of OPN 
      and OC, and DNA synthesis in HPL cells were examined. RESULTS: NT-4/5 and trkB 
      mRNA were expressed in HPL cells. NT-4/5 elevated the mRNA levels of ALPase, OPN, 
      OC, and BMP-2 and the syntheses of OPN, OC, and DNA in HPL cells. PD98059, an 
      extracellular signal-regulated kinase (ERK) inhibitor, obviated the increase in 
      the mRNA levels of ALPase, OPN, OC, and BMP-2. NT-4/5 increased the levels of 
      phosphorylated ERK1/2. Furthermore, NT-4/5 enhanced the amount of mineral 
      deposits in cultures of HPL cells. CONCLUSION: NT-4/5, as well as BDNF and NGF, 
      is suggested to play a role in the regulation of function of periodontal ligament 
      cells.
FAU - Mizuno, Noriyoshi
AU  - Mizuno N
AD  - Department of Periodontal Medicine, Hiroshima University Graduate School of 
      Biomedical Sciences, Hiroshima, Japan. mizuno@hiroshima-u.ac.jp
FAU - Shiba, Hideki
AU  - Shiba H
FAU - Inui, Takafumi
AU  - Inui T
FAU - Takeda, Katsuhiro
AU  - Takeda K
FAU - Kajiya, Mikihito
AU  - Kajiya M
FAU - Hasegawa, Naohiko
AU  - Hasegawa N
FAU - Kawaguchi, Hiroyuki
AU  - Kawaguchi H
FAU - Kurihara, Hidemi
AU  - Kurihara H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - 106441-73-0 (Osteopontin)
RN  - 145172-44-7 (neurotrophin 5)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Alkaline Phosphatase/metabolism
MH  - Bone Morphogenetic Protein 2/metabolism
MH  - Bone and Bones/metabolism
MH  - Cell Differentiation/*physiology
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - DNA/biosynthesis
MH  - Dental Cementum/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Humans
MH  - Male
MH  - Nerve Growth Factors/genetics/*metabolism
MH  - Osteopontin/metabolism
MH  - Periodontal Ligament/cytology/*metabolism
MH  - RNA, Messenger/analysis
MH  - Receptor, trkB/genetics/metabolism
MH  - Young Adult
EDAT- 2008/11/05 09:00
MHDA- 2009/02/13 09:00
CRDT- 2008/11/05 09:00
PHST- 2008/11/05 09:00 [pubmed]
PHST- 2009/02/13 09:00 [medline]
PHST- 2008/11/05 09:00 [entrez]
AID - 10.1902/jop.2008.070402 [doi]
PST - ppublish
SO  - J Periodontol. 2008 Nov;79(11):2182-9. doi: 10.1902/jop.2008.070402.