PMID- 18980615
OWN - NLM
STAT- MEDLINE
DCOM- 20090529
LR  - 20220321
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Print)
IS  - 0041-1132 (Linking)
VI  - 49
IP  - 2
DP  - 2009 Feb
TI  - Long-term in vitro reactivity for human leukocyte antigen antibodies and 
      comparison of detection using serum versus plasma.
PG  - 243-51
LID - 10.1111/j.1537-2995.2008.01955.x [doi]
AB  - BACKGROUND: Human leukocyte antigen (HLA) antibodies are a possible cause of 
      transfusion-related acute lung injury (TRALI), and fluorescent bead assays are 
      often used for antibody detection. Serum is the manufacturer's recommended 
      sample, but plasma may be easier to obtain for studies of HLA antibody prevalence 
      and TRALI case investigations. STUDY DESIGN AND METHODS: Specimens were obtained 
      from 44 multiparous females positive for the presence of HLA antibodies by 
      lymphocytotoxicity testing at least 13 years prior and from 1000 contemporary 
      blood donors. Screening tests were performed using a multiplex bead-based assay. 
      In addition to comparing results obtained with paired plasma and serum samples, 
      the effects of storage at 4 degrees C for 1 week and of multiple freeze-thaw 
      cycles were evaluated. RESULTS: Of 42 evaluable subjects with HLA antibodies 
      documented more than 13 years earlier, only 1 showed loss of detectable 
      antibodies, with 39 (93%) positive in the screening assay for HLA Class I and 24 
      (57%) positive in the screening assay for HLA Class II antibodies. In 968 
      evaluable contemporary donors, 291 screened positive for the presence of HLA 
      Class I and 206 for HLA Class II antibodies using a low assay cutoff. Screening 
      test concordance using paired plasma and serum samples was high, particularly for 
      subjects with higher-level antibodies. Refrigeration of samples for 1 week did 
      not significantly affect assay results, while repeated freeze-thaw cycles caused 
      a decrement in signal level. CONCLUSION: Serum and plasma samples gave concordant 
      results in the majority of cases, particularly for specimens with higher-level 
      antibodies. High-level HLA antibodies were present in most individuals for more 
      than 13 years.
FAU - Norris, Philip J
AU  - Norris PJ
AD  - Blood Systems Research Institute, University of California, San Francisco, USA. 
      pnorris@bloodsystems.org
FAU - Lee, Jar-How
AU  - Lee JH
FAU - Carrick, Danielle M
AU  - Carrick DM
FAU - Gottschall, Jerome L
AU  - Gottschall JL
FAU - Lebedeva, Mila
AU  - Lebedeva M
FAU - de Castro, B R
AU  - de Castro BR
FAU - Kleinman, Steven H
AU  - Kleinman SH
FAU - Busch, Michael P
AU  - Busch MP
LA  - eng
GR  - N01-HB-47172/HB/NHLBI NIH HHS/United States
GR  - N01-HB-47171/HB/NHLBI NIH HHS/United States
GR  - HHSN268200517181C/PHS HHS/United States
GR  - N01-HB-47170/HB/NHLBI NIH HHS/United States
GR  - N01-HB-47175/HB/NHLBI NIH HHS/United States
GR  - N01HB57181/HL/NHLBI NIH HHS/United States
GR  - N01-HB-47174/HB/NHLBI NIH HHS/United States
GR  - N01-HB-47168/HB/NHLBI NIH HHS/United States
GR  - N01-HB-57181/HB/NHLBI NIH HHS/United States
GR  - N01-HB-47169/HB/NHLBI NIH HHS/United States
GR  - N01HB47168/HL/NHLBI NIH HHS/United States
GR  - N01-HB-47173/HB/NHLBI NIH HHS/United States
GR  - HHSN268200517181C/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20081029
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Antibodies)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Acute Lung Injury/etiology
MH  - Antibodies/*immunology
MH  - Blood Donors
MH  - Cohort Studies
MH  - Cytotoxicity Tests, Immunologic
MH  - Female
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Histocompatibility Antigens Class II/*immunology
MH  - Humans
MH  - Parity
MH  - Plasma/*immunology
MH  - Pregnancy
MH  - Prevalence
MH  - Reproducibility of Results
MH  - Serum/*immunology
MH  - Time Factors
MH  - United States/epidemiology
PMC - PMC3058293
MID - NIHMS88843
EDAT- 2008/11/05 09:00
MHDA- 2009/05/30 09:00
PMCR- 2011/03/16
CRDT- 2008/11/05 09:00
PHST- 2008/11/05 09:00 [pubmed]
PHST- 2009/05/30 09:00 [medline]
PHST- 2008/11/05 09:00 [entrez]
PHST- 2011/03/16 00:00 [pmc-release]
AID - TRF01955 [pii]
AID - 10.1111/j.1537-2995.2008.01955.x [doi]
PST - ppublish
SO  - Transfusion. 2009 Feb;49(2):243-51. doi: 10.1111/j.1537-2995.2008.01955.x. Epub 
      2008 Oct 29.