PMID- 18980769
OWN - NLM
STAT- MEDLINE
DCOM- 20090219
LR  - 20191210
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1213
IP  - 2
DP  - 2008 Dec 12
TI  - Enhanced analysis of triterpenes, flavonoids and phenolic compounds in Prunella 
      vulgaris L. by capillary zone electrophoresis with the addition of running buffer 
      modifiers.
PG  - 231-8
LID - 10.1016/j.chroma.2008.10.033 [doi]
AB  - A cyclodextrin-modified capillary zone electrophoresis method was developed for 
      the separation and determination of three isomeric compounds (ursolic acid, 
      oleanolic acid and betulinic acid), caffeic acid, p-coumaric acid, rosmarinic 
      acid, rutin and quercetin. Without the addition of beta-cyclodextrin (beta-CD) 
      and methanol, the separation of these analytes was poorly resolved. These eight 
      compounds, however, were well separated from each other within 20 min with a 
      borax running buffer (40 mM of borax, pH 9.4) containing 2mM beta-CD and 4% (v/v) 
      methanol at the voltage of 25 kV, temperature of 25 degrees C and detection 
      wavelength of 210 nm. The relative standard deviations (RSDs) of migration time 
      ranged from 0.16 to 0.74% while those of the peak area ratios ranged from 2.17 to 
      4.61% for six determinations of the analytes at concentration of 10 and 25 microg 
      mL(-1). The correlation coefficients of the calibration curves of the analytes 
      were all >0.998, and the recoveries were from 96.8 to 103.6%. The method was 
      successfully applied to determine these bioactive components in the samples of 
      Prunella vulgaris L. and its beverage drink products. Our results reveal that 
      only the isomeric compounds and rosmarinic acid could be detected in the spikes 
      of P. vulgaris L.; other components were either too low to be detected or not 
      present while only rosmarinic acid was detected in the beverage products.
FAU - Cheung, Hon-Yeung
AU  - Cheung HY
AD  - Research Group for Bioactive Products, Department of Biology and Chemistry, City 
      University of Hong Kong, 83 Tat Chee Avenue, Hong Kong, SAR, China. 
      bhhonyun@cityu.edu.hk
FAU - Zhang, Qing-Feng
AU  - Zhang QF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20081015
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Buffers)
RN  - 0 (Flavonoids)
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
RN  - 0 (Triterpenes)
SB  - IM
MH  - Buffers
MH  - Electrophoresis, Capillary/methods
MH  - Flavonoids/*analysis
MH  - Hydrogen-Ion Concentration
MH  - Phenols/*analysis
MH  - Plant Extracts/analysis
MH  - Prunella/*chemistry
MH  - Reproducibility of Results
MH  - Triterpenes/*analysis
EDAT- 2008/11/05 09:00
MHDA- 2009/02/20 09:00
CRDT- 2008/11/05 09:00
PHST- 2008/07/20 00:00 [received]
PHST- 2008/10/08 00:00 [revised]
PHST- 2008/10/09 00:00 [accepted]
PHST- 2008/11/05 09:00 [pubmed]
PHST- 2009/02/20 09:00 [medline]
PHST- 2008/11/05 09:00 [entrez]
AID - S0021-9673(08)01781-0 [pii]
AID - 10.1016/j.chroma.2008.10.033 [doi]
PST - ppublish
SO  - J Chromatogr A. 2008 Dec 12;1213(2):231-8. doi: 10.1016/j.chroma.2008.10.033. 
      Epub 2008 Oct 15.