PMID- 18981655
OWN - NLM
STAT- MEDLINE
DCOM- 20090108
LR  - 20190606
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 70
IP  - 10
DP  - 2008 Oct
TI  - Efficacy of vaccination with porcine reproductive and respiratory syndrome virus 
      following challenges with field isolates in Japan.
PG  - 1017-25
AB  - The objective of this study was to evaluate the influences of genetic and 
      antigenic variations in field isolates of porcine reproductive and respiratory 
      syndrome virus (PRRSV) on vaccine efficacy. Four-week-old pigs were vaccinated 
      with a commercial modified live virus vaccine. Four weeks after vaccination, pigs 
      in both the vaccinated group and the non-vaccinated group were challenged 
      intranasally with 10(7) TCID(50) of PRRSV wt-11 (Experiment 1) or PRRSV wt-7 
      (Experiment 2). Based on genome sequencing of ORF5 and cross neutralization test 
      results, PRRSV wt-11 is similar to the vaccine strain, whereas wt-7 is distinct 
      from the vaccine strain. In the vaccinated challenged groups, clinical signs were 
      less severe, the mean rate of weight gain was greater, and gross lung lesions 
      were less severe when compared with the non-vaccinated challenged groups in both 
      experiments. In Experiment 1, the virus was isolated from serum at 3 days 
      post-challenge, and the mean virus titers in broncho-alveolar lavage fluids 
      (BALF) and tissues were lower in pigs in the vaccinated challenged groups 
      compared with those in the non-vaccinated challenged group. In Experiment 2, 
      virus isolation from serum, BALF and tissues showed no significant differences 
      between the groups. These results suggest that commercial PRRSV vaccine could be 
      effective in reducing clinical disease following a challenge with field isolates 
      of PRRSV. However, with regards to virological protection, the efficacy of the 
      vaccine may be affected by the nature of the PRRSV isolates.
FAU - Okuda, Yo
AU  - Okuda Y
AD  - JA Zen-noh (National Federation of Agricultural Cooperative Associations), 
      Institute of Animal Health, Sakura, Chiba, Japan. okuda-you@zennoh.or.jp
FAU - Kuroda, Maho
AU  - Kuroda M
FAU - Ono, Masaaki
AU  - Ono M
FAU - Chikata, Sumiko
AU  - Chikata S
FAU - Shibata, Isao
AU  - Shibata I
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Antigenic Variation
MH  - Body Temperature
MH  - Body Weight
MH  - Bronchoalveolar Lavage Fluid/virology
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Leukocyte Count/veterinary
MH  - Lung/virology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/immunology/prevention & 
      control/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - Specific Pathogen-Free Organisms
MH  - Swine
MH  - Vaccination/*veterinary
MH  - Viral Vaccines/immunology/*therapeutic use
EDAT- 2008/11/05 09:00
MHDA- 2009/01/09 09:00
CRDT- 2008/11/05 09:00
PHST- 2008/11/05 09:00 [pubmed]
PHST- 2009/01/09 09:00 [medline]
PHST- 2008/11/05 09:00 [entrez]
AID - JST.JSTAGE/jvms/70.1017 [pii]
AID - 10.1292/jvms.70.1017 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2008 Oct;70(10):1017-25. doi: 10.1292/jvms.70.1017.