PMID- 18981663
OWN - NLM
STAT- MEDLINE
DCOM- 20090108
LR  - 20190606
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 70
IP  - 10
DP  - 2008 Oct
TI  - Impaired expression of excitatory amino acid transporter 2 (EAAT2) and glutamate 
      homeostasis in canine necrotizing meningoencephalitis.
PG  - 1071-5
AB  - To clarify the involvement of excitatory and inhibitory amino acids in canine 
      necrotizing meningoencephalitis (NME), glutamate, aspartate, taurine and 
      gamma-aminobutylic acid (GABA) were determined in the cerebrospinal fluids (CSF) 
      from eight NME cases and ten healthy controls. NME dogs exhibited significantly 
      higher concentrations of glutamate and aspartate than those in controls (p<0.001 
      and p<0.001, respectively), while there was no difference in taurine or GABA 
      between the two groups. When fetal canine astrocytes were cultured for 24 hr in 
      the presence of NME-CSF, supernatant concentrations of glutamate, aspartate and 
      taurine were significantly elevated. Simultaneously, expression of excitatory 
      amino acid transporter 2 (EAAT2) mRNA was significantly reduced in the astrocytes 
      without change in EAAT1 mRNA. Hence, reduced expression of EAAT2 and impaired 
      glutamate homeostasis may contribute to the pathogenesis of NME.
FAU - Pham, Ngoc-Thi
AU  - Pham NT
AD  - Laboratory of Veterinary Clinical Pathobiology, Department of Veterinary Medical 
      Sciences, Graduate School of Agricultural and Life Sciences, The University of 
      Tokyo, Bunkyo-ku, Tokyo, Japan.
FAU - Matsuki, Naoaki
AU  - Matsuki N
FAU - Shibuya, Masahiro
AU  - Shibuya M
FAU - Tamahara, Satoshi
AU  - Tamahara S
FAU - Ono, Kenichiro
AU  - Ono K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Excitatory Amino Acid Transporter 1)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 1EQV5MLY3D (Taurine)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Animals
MH  - Aspartic Acid/metabolism
MH  - Astrocytes/chemistry/metabolism
MH  - Dog Diseases/cerebrospinal fluid/*metabolism
MH  - Dogs
MH  - Excitatory Amino Acid Transporter 1/biosynthesis/genetics
MH  - Excitatory Amino Acid Transporter 2/*biosynthesis/genetics
MH  - Female
MH  - Glutamic Acid/*metabolism
MH  - Leukoencephalitis, Acute Hemorrhagic/cerebrospinal fluid/metabolism/*veterinary
MH  - Male
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Taurine/metabolism
MH  - Tumor Necrosis Factor-alpha/cerebrospinal fluid
MH  - gamma-Aminobutyric Acid/metabolism
EDAT- 2008/11/05 09:00
MHDA- 2009/01/09 09:00
CRDT- 2008/11/05 09:00
PHST- 2008/11/05 09:00 [pubmed]
PHST- 2009/01/09 09:00 [medline]
PHST- 2008/11/05 09:00 [entrez]
AID - JST.JSTAGE/jvms/70.1071 [pii]
AID - 10.1292/jvms.70.1071 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2008 Oct;70(10):1071-5. doi: 10.1292/jvms.70.1071.