PMID- 18983758
OWN - NLM
STAT- MEDLINE
DCOM- 20090128
LR  - 20190917
IS  - 1472-6491 (Electronic)
IS  - 1472-6483 (Linking)
VI  - 17
IP  - 5
DP  - 2008 Nov
TI  - Stability of placental RNA using dried maternal blood spots.
PG  - 716-21
AB  - Having demonstrated successful recovery and detection of placental transcripts 
      from dried blood spots (DBS), various preanalytical conditions were examined to 
      determine optimal handling of samples. The role of several factors was explored, 
      including temperature (4 degrees C versus 25 degrees C), processing time (24 h to 
      8 weeks), and addition of preservatives (RNA later and formalin) that may 
      interfere with stability and detection of placental transcripts in DBS. mRNA 
      transcripts encoding human glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 
      internal control) and beta-human chorionic gonadotrophin (beta HCG; placental) 
      were analysed by real-time-polymerase chain reaction using DBS from 23 pregnant 
      women. GAPDH and beta HCG transcripts were detected in all samples 24 h after 
      collection. Although treatment of blood with RNA later did not affect RNA 
      recovery, formalin treatment negatively affected RNA recovery from DBS. 
      Temperature did not have a significant effect on levels of either transcript. 
      Storage time caused a significant decrease in GAPDH after 4 weeks (P = 0.014) and 
      beta HCG after 1 week (P = 0.007). Decrease of beta HCG levels after 1 week 
      followed by steady detectable levels for up to 4 weeks suggests two populations 
      of circulating placental transcript exist, a population susceptible to 
      degradation in blood versus a more stable form. Therefore, defining proper 
      parameters for collection and storage of DBS further reinforces reliable analysis 
      of target sequences for clinical testing.
FAU - Jorgez, Carolina J
AU  - Jorgez CJ
AD  - Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, TX, 
      USA.
FAU - Bischoff, Farideh Z
AU  - Bischoff FZ
LA  - eng
GR  - HDO46623/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - Netherlands
TA  - Reprod Biomed Online
JT  - Reproductive biomedicine online
JID - 101122473
RN  - 0 (Chorionic Gonadotropin, beta Subunit, Human)
RN  - 0 (RNA, Messenger)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Chorionic Gonadotropin, beta Subunit, Human/genetics
MH  - Female
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
MH  - Humans
MH  - Placenta/*metabolism
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - RNA Stability
MH  - RNA, Messenger/*blood/genetics/isolation & purification
EDAT- 2008/11/06 09:00
MHDA- 2009/01/29 09:00
CRDT- 2008/11/06 09:00
PHST- 2008/11/06 09:00 [pubmed]
PHST- 2009/01/29 09:00 [medline]
PHST- 2008/11/06 09:00 [entrez]
AID - S1472-6483(10)60321-5 [pii]
AID - 10.1016/s1472-6483(10)60321-5 [doi]
PST - ppublish
SO  - Reprod Biomed Online. 2008 Nov;17(5):716-21. doi: 10.1016/s1472-6483(10)60321-5.