PMID- 1898611
OWN - NLM
STAT- MEDLINE
DCOM- 19910125
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 49
IP  - 1
DP  - 1991 Jan
TI  - Effects of TNF alpha on the expression of class II MHC molecules in macrophages 
      induced by IFN gamma: evidence for suppression at the level of transcription.
PG  - 21-8
AB  - Tumor necrosis factor-alpha (TNF-alpha) induces surface expression of class II 
      major histocompatibility (MHC) molecules (la molecules) in many cells, including 
      macrophage-like cell lines. When we tested the effects of this cytokine on murine 
      peritoneal macrophages, TNF alpha had little effect on surface expression of la. 
      The strong expression of such molecules induced by interferon-gamma (IFN gamma) 
      was, however, suppressed moderately by TNF alpha. These effects were reflected at 
      the level of specific messenger RNA (mRNA) as detected by Northern blot analysis. 
      Furthermore, the locus of control appears to be transcriptional; in nuclear 
      run-on assays, TNF alpha suppressed the IFN gamma-induced enhancement of 
      transcription for the murine beta-chain of I-A (I-A beta.). Taken together the 
      data suggest that TNF alpha has little effect on class II MHC genes and surface 
      expression in murine peritoneal macrophages, that TNF alpha is a modest 
      suppressant of such molecules when their levels are raised by IFN gamma, and that 
      these suppressive effects are mediated at the level of transcription.
FAU - Melhus, O
AU  - Melhus O
AD  - Department of Pathology, Duke University Medical Center, Durham, North Carolina 
      27710.
FAU - Koerner, T J
AU  - Koerner TJ
FAU - Adams, D O
AU  - Adams DO
LA  - eng
GR  - CA 16784/CA/NCI NIH HHS/United States
GR  - CA 29586/CA/NCI NIH HHS/United States
GR  - ES 02922/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Gene Expression Regulation/drug effects
MH  - Histocompatibility Antigens Class II/*biosynthesis/genetics
MH  - Interferon-gamma/*pharmacology
MH  - Macrophages/drug effects/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger/analysis
MH  - Transcription, Genetic/*drug effects
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1002/jlb.49.1.21 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1991 Jan;49(1):21-8. doi: 10.1002/jlb.49.1.21.