PMID- 18987582
OWN - NLM
STAT- MEDLINE
DCOM- 20090219
LR  - 20131121
IS  - 0172-780X (Print)
IS  - 0172-780X (Linking)
VI  - 29
IP  - 5
DP  - 2008 Oct
TI  - Screening and semiquantitative analysis of drugs and drugs of abuse in human 
      serum samples using gas chromatography-mass spectrometry.
PG  - 749-54
AB  - OBJECTIVES: The purpose of this study is to develop the gas chromatographic-mass 
      spectrometric method (GC-MS) for screening and semiquantification of drugs and 
      drugs of abuse in human serum. METHOD: GC-MS method after liquid-liquid 
      extraction (LLE) and derivatization with 
      N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) is presented for screening as 
      well as identification and semiquantification of the most frequently used drugs 
      and drugs of abuse in human serum. RESULTS: Bovine serum spiked with ephedrine 
      (EPHE), 3,4-methylenedioxymethamphetamine (MDMA), guaifenesin (GUAIF), tramadol 
      (TRAM), phenobarbital (PHENO), amitriptyline (AMITR), cocaine (COCA), mirtazapine 
      (MIRTA), dothiepin (DOTH), citalopram (CITAL), clomipramine (CLOMI), bromazepam 
      (BMZPM), diazepam (DZPM), codeine (COD), morphine (MORPH), levomepromazine 
      (LEVO), zolpidem (ZOLP), clozapine (CLOZP), alprazolam (ALPZM) was used for the 
      recovery and repeatability study and for preparation of calibration curves of 
      individual compounds or their TMS derivatives. Recoveries were tested on 
      concentration levels 0.05, 0.1 and 0.5 microg/mL (n=6) and established in range 
      72.0-98.0%. Repeatabilities expressed as relative standard deviations (RSDs) 
      measured at concentration levels 0.05, 0.1 and 0.5 microg/ mL (n=6) were lower 
      than 10.0%. The calibration curves for analytes or their TMS derivatives were 
      linear in concentration range 0.025-2.000 microg/mL (except of EPHE 2TMS, MDMA 
      TMS, MORPH 2TMS, BMZPM TMS, ALPZM) with correlation coefficients exceeding 0.99. 
      The limit of quantification (LOQ) for analytes used for evaluation study was 
      0.025 microg/mL (except analytes mentioned above). CONCLUSIONS: The GC-MS method 
      presented here is allowing screening, identification and semiquantification of 
      the most commonly encountered drugs and drugs of abuse in human serum and can be 
      successfully applied to analysis of real samples from clinical and forensic 
      toxicology cases.
FAU - Maresova, Vera
AU  - Maresova V
AD  - Institute of Forensic Medicine and Toxicology, First Faculty of Medicine, Charles 
      University in Prague, Prague, Czech Republic. vera.maresova@lf1.cuni.cz
FAU - Chadt, Jiri
AU  - Chadt J
FAU - Novakova, Eva
AU  - Novakova E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Sweden
TA  - Neuro Endocrinol Lett
JT  - Neuro endocrinology letters
JID - 8008373
RN  - 0 (Acetamides)
RN  - 0 (Fluoroacetates)
RN  - 0 (Pharmaceutical Preparations)
RN  - 0 (Trimethylsilyl Compounds)
RN  - 24589-78-4 (N-methyl-N-(trimethylsilyl)trifluoroacetamide)
RN  - E5R8Z4G708 (Trifluoroacetic Acid)
SB  - IM
MH  - Acetamides
MH  - Calibration
MH  - Fluoroacetates
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Pharmaceutical Preparations/*blood
MH  - Reproducibility of Results
MH  - Solid Phase Extraction
MH  - Substance Abuse Detection/*methods
MH  - Substance-Related Disorders/*blood
MH  - Trifluoroacetic Acid/chemistry
MH  - Trimethylsilyl Compounds/chemistry
EDAT- 2008/11/07 09:00
MHDA- 2009/02/20 09:00
CRDT- 2008/11/07 09:00
PHST- 2008/06/30 00:00 [received]
PHST- 2008/09/05 00:00 [accepted]
PHST- 2008/11/07 09:00 [pubmed]
PHST- 2009/02/20 09:00 [medline]
PHST- 2008/11/07 09:00 [entrez]
AID - NEL290508A28 [pii]
PST - ppublish
SO  - Neuro Endocrinol Lett. 2008 Oct;29(5):749-54.