PMID- 18987950
OWN - NLM
STAT- MEDLINE
DCOM- 20090415
LR  - 20191210
IS  - 0957-4530 (Print)
IS  - 0957-4530 (Linking)
VI  - 20
IP  - 3
DP  - 2009 Mar
TI  - Evaluation of alginate-chitosan semi IPNs as cartilage scaffolds.
PG  - 699-709
LID - 10.1007/s10856-008-3624-x [doi]
AB  - In this study, alginate and alginate:chitosan semi interpenetrating polymer 
      network (IPN) scaffolds were prepared by freeze-drying process. Alginate 
      scaffolds were crosslinked with different concentrations of CaCl(2), i.e. 0.5, 1 
      or 3% (w/v), in 96% (v/v) ethanol solutions for two different periods, i.e. 4 and 
      24 h, after freeze-drying. Scanning electron microscope (SEM)/ Energy Dispersive 
      Analysis by X-ray (EDAX) analysis and swelling studies indicated that 
      crosslinking of scaffolds with 3% (w/v) CaCl(2) for 24 h was effectively created 
      suitable alginate scaffolds in terms of optimum porosity and mechanical 
      stability. This is why, alginate:chitosan semi IPN scaffolds were prepared at the 
      crosslinking condition mentioned above in 70:30, 60:40 and 50:50% (v/v) 
      alginate:chitosan ratios. Besides the attachment and proliferation abilities of 
      ATDC5 murine chondrogenic cells on alginate, 70:30% (v/v) alginate:chitosan and 
      50:50% (v/v) alginate:chitosan scaffolds, their cellular responses were assessed 
      for chondrogenic potential. These structural and cellular outcomes demonstrate 
      potential utility of chitosan semi IPNs in alginate scaffolds. Comparative 
      results found in relation to alginate scaffolds, support the necessity for 
      alginate:chitosan scaffolds for improved cartilage tissue engineering.
FAU - Tigli, R Seda
AU  - Tigli RS
AD  - Chemical Engineering Department, Hacettepe University, Beytepe, 06800, Ankara, 
      Turkey.
FAU - Gumusderelioglu, Menemse
AU  - Gumusderelioglu M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20081106
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 0 (Alginates)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Hexuronic Acids)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Alginates/*chemistry
MH  - Animals
MH  - Cell Adhesion
MH  - Cell Line
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Chitosan/*chemistry
MH  - Chondrocytes/cytology
MH  - Chondrogenesis
MH  - Cross-Linking Reagents
MH  - Freeze Drying
MH  - Glucuronic Acid/chemistry
MH  - Hexuronic Acids/chemistry
MH  - Materials Testing
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
EDAT- 2008/11/07 09:00
MHDA- 2009/04/16 09:00
CRDT- 2008/11/07 09:00
PHST- 2008/06/11 00:00 [received]
PHST- 2008/10/15 00:00 [accepted]
PHST- 2008/11/07 09:00 [pubmed]
PHST- 2009/04/16 09:00 [medline]
PHST- 2008/11/07 09:00 [entrez]
AID - 10.1007/s10856-008-3624-x [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2009 Mar;20(3):699-709. doi: 10.1007/s10856-008-3624-x. 
      Epub 2008 Nov 6.