PMID- 1898883
OWN - NLM
STAT- MEDLINE
DCOM- 19910221
LR  - 20181130
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 128
IP  - 1
DP  - 1991 Jan
TI  - Evidence that secretion of immunoactive inhibin by seminiferous tubules from the 
      adult rat testis is regulated by specific germ cell types: correlation between in 
      vivo and in vitro studies.
PG  - 467-76
AB  - This study has assessed whether depletion of specific germ cell types is able to 
      alter the secretion of immunoactive inhibin by adult Sertoli cells in vivo and in 
      vitro. Pachytene and later spermatocytes were depleted (80-100%) by a single 
      administration of methoxy acetic acid (MAA; 650 mg/kg) to adult rats. At 
      intervals between 1 and 42 days posttreatment, rats were killed, and the blood 
      levels of FSH, LH, and testosterone were determined together with the levels of 
      immunoactive inhibin in plasma and testicular interstitial fluid (IF). At the 
      same time intervals, seminiferous tubules (ST; 5 x 2 cm) were isolated from 
      control and MAA-treated rats and cultured for 24-72 h in the presence or absence 
      of rat FSH, (Bu)2cAMP, or MAA under rigorously optimized conditions. The hormonal 
      changes observed were related to the presence/absence of specific germ cell 
      types, as determined by assessment of testicular morphology in perfusion-fixed 
      testes from similarly treated rats. One to 3 days after MAA treatment, coincident 
      with the depletion of pachytene spermatocytes, blood levels of FSH were increased 
      significantly compared with controls; FSH returned to control levels at 7-14 days 
      (when early spermatids were depleted), but were increased again at 21-35 days 
      (when late spermatids were depleted). In contrast, while the plasma levels of 
      immunoactive inhibin were increased 2-fold 3 days posttreatment, they were 
      comparable to controls at 7-14 days, but were decreased substantially at 21-28 
      days. The levels of immunoactive inhibin in testicular IF were more than doubled 
      1 and 3 days posttreatment, but were comparable to control levels at all other 
      times. Blood levels of LH showed a similar pattern of change to FSH, although 
      only at 21-28 days after MAA treatment was there a significant increase, while 
      blood levels of testosterone were comparable at all times in control and 
      MAA-treated rats. To confirm that the changes observed in vivo after MAA 
      treatment were indicative of changes in Sertoli rather than Leydig cell secretion 
      of immonoactive inhibin, its secretion by isolated ST was assessed, and a pattern 
      of change similar to that in plasma was observed. Thus, when cultured for 24 h 
      under basal conditions, ST from rats 1-3 days after MAA treatment showed a 2- to 
      3-fold increase in secretion of immunoactive inhibin, which returned to control 
      levels at 7-14 days before being reduced substantially at 21-28 days and then 
      recovering to control levels; similar changes were observed for FSH- and 
      (Bu)2cAMP-stimulated secretion of immunoactive inhibin.(ABSTRACT TRUNCATED AT 400 
      WORDS)
FAU - Allenby, G
AU  - Allenby G
AD  - Medical Research Council Reproductive Biology Unit, Centre for Reproductive 
      Biology, Edinburgh, Scotland.
FAU - Foster, P M
AU  - Foster PM
FAU - Sharpe, R M
AU  - Sharpe RM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Acetates)
RN  - 57285-09-3 (Inhibins)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - F11T1H7Q7W (methoxyacetic acid)
SB  - IM
MH  - Acetates/pharmacology
MH  - Animals
MH  - Follicle Stimulating Hormone/blood
MH  - Inhibins/blood/immunology/*metabolism
MH  - Luteinizing Hormone/blood
MH  - Male
MH  - Organ Culture Techniques
MH  - Radioimmunoassay
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Reference Values
MH  - Seminiferous Tubules/*metabolism
MH  - Spermatocytes/drug effects/physiology
MH  - Spermatozoa/cytology/*physiology
MH  - Testis/cytology/*physiology
MH  - Time Factors
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1210/endo-128-1-467 [doi]
PST - ppublish
SO  - Endocrinology. 1991 Jan;128(1):467-76. doi: 10.1210/endo-128-1-467.