PMID- 18989442
OWN - NLM
STAT- MEDLINE
DCOM- 20090204
LR  - 20181113
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 7
DP  - 2007
TI  - A gradient-generating microfluidic device for cell biology.
PG  - 271
LID - 10.3791/271 [doi]
LID - 271
AB  - The fabrication and operation of a gradient-generating microfluidic device for 
      studying cellular behavior is described. A microfluidic platform is an enabling 
      experimental tool, because it can precisely manipulate fluid flows, enable 
      high-throughput experiments, and generate stable soluble concentration gradients. 
      Compared to conventional gradient generators, poly(dimethylsiloxane) (PDMS)-based 
      microfluidic devices can generate stable concentration gradients of growth 
      factors with well-defined profiles. Here, we developed simple gradient-generating 
      microfluidic devices with three separate inlets. Three microchannels combined 
      into one microchannel to generate concentration gradients. The stability and 
      shape of growth factor gradients were confirmed by fluorescein isothyiocyanate 
      (FITC)-dextran with a molecular weight similar to epidermal growth factor (EGF). 
      Using this microfluidic device, we demonstrated that fibroblasts exposed to 
      concentration gradients of EGF migrated toward higher concentrations. The 
      directional orientation of cell migration and motility of migrating cells were 
      quantitatively assessed by cell tracking analysis. Thus, this gradient-generating 
      microfluidic device might be useful for studying and analyzing the behavior of 
      migrating cells.
FAU - Chung, Bong Geun
AU  - Chung BG
AD  - Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute 
      of Technology, Center for Biomedical Engineering, Department of Medicine, Brigham 
      and Women's Hospital, Boston, MA, USA.
FAU - Manbachi, Amir
AU  - Manbachi A
FAU - Saadi, Wajeeh
AU  - Saadi W
FAU - Lin, Francis
AU  - Lin F
FAU - Jeon, Noo Li
AU  - Jeon NL
FAU - Khademhosseini, Ali
AU  - Khademhosseini A
LA  - eng
PT  - Journal Article
PT  - Video-Audio Media
DEP - 20070830
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Dextrans)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (fluorescein isothiocyanate dextran)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - *Cell Physiological Phenomena
MH  - Cells/drug effects/metabolism
MH  - Dextrans/chemistry
MH  - Epidermal Growth Factor/chemistry/pharmacology
MH  - Equipment Design
MH  - Fibroblasts/metabolism
MH  - Fluorescein-5-isothiocyanate/analogs & derivatives/chemistry
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/metabolism
MH  - Microfluidic Analytical Techniques/*instrumentation
MH  - Molecular Weight
MH  - Osmolar Concentration
PMC - PMC2565846
EDAT- 2008/11/08 09:00
MHDA- 2009/02/05 09:00
PMCR- 2009/08/30
CRDT- 2008/11/08 09:00
PHST- 2008/11/08 09:00 [pubmed]
PHST- 2009/02/05 09:00 [medline]
PHST- 2008/11/08 09:00 [entrez]
PHST- 2009/08/30 00:00 [pmc-release]
AID - 271 [pii]
AID - 10.3791/271 [doi]
PST - ppublish
SO  - J Vis Exp. 2007;(7):271. doi: 10.3791/271. Epub 2007 Aug 30.