PMID- 18989461
OWN - NLM
STAT- MEDLINE
DCOM- 20090105
LR  - 20181113
IS  - 1553-7374 (Electronic)
IS  - 1553-7366 (Print)
IS  - 1553-7366 (Linking)
VI  - 4
IP  - 11
DP  - 2008 Nov
TI  - Urea-mediated cross-presentation of soluble Epstein-Barr virus BZLF1 protein.
PG  - e1000198
LID - 10.1371/journal.ppat.1000198 [doi]
LID - e1000198
AB  - Soluble extracellular proteins usually do not enter the endogenous human 
      leukocyte antigen (HLA) I-dependent presentation pathway of antigen-presenting 
      cells, strictly impeding their applicability for the re-stimulation of 
      protein-specific CD8(+) cytotoxic T lymphocytes (CTL). Here we present for the 
      Epstein-Barr virus (EBV) BZLF1 a novel strategy that facilitates protein 
      translocation into antigen-presenting cells by its solubilisation in high molar 
      urea and subsequent pulsing of cells in presence of low molar urea. Stimulation 
      of PBMC from HLA-matched EBV-seropositive individuals with urea-treated BZLF1 but 
      not untreated BZLF1 induces an efficient reactivation of BZLF1-specific CTL. 
      Urea-treated BZLF1 (uBZLF1) enters antigen-presenting cells in a 
      temperature-dependent manner by clathrin-mediated endocytosis and is processed by 
      the proteasome into peptides that are bound to nascent HLA I molecules. Dendritic 
      cells and monocytes but also B cells can cross-present uBZLF1 in vitro. The 
      strategy described here has potential for use in the development of improved 
      technologies for the monitoring of protein-specific CTL.
FAU - Barabas, Sascha
AU  - Barabas S
AD  - Institute of Medical Microbiology, University of Regensburg, Germany.
FAU - Gary, Regina
AU  - Gary R
FAU - Bauer, Tanja
AU  - Bauer T
FAU - Lindner, Juha
AU  - Lindner J
FAU - Lindner, Petra
AU  - Lindner P
FAU - Weinberger, Birgit
AU  - Weinberger B
FAU - Jilg, Wolfgang
AU  - Jilg W
FAU - Wolf, Hans
AU  - Wolf H
FAU - Deml, Ludwig
AU  - Deml L
LA  - eng
SI  - RefSeq/YP_401673
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081107
PL  - United States
TA  - PLoS Pathog
JT  - PLoS pathogens
JID - 101238921
RN  - 0 (BZLF1 protein, Herpesvirus 4, Human)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Trans-Activators)
RN  - 8W8T17847W (Urea)
SB  - IM
MH  - Antigen-Presenting Cells/immunology/virology
MH  - B-Lymphocytes
MH  - Cells, Cultured
MH  - Cross-Priming/*immunology
MH  - Dendritic Cells
MH  - Endocytosis
MH  - Herpesvirus 4, Human/*chemistry
MH  - Histocompatibility Antigens Class I
MH  - Humans
MH  - Methods
MH  - Monocytes
MH  - Solubility
MH  - Trans-Activators/*immunology
MH  - *Urea
PMC - PMC2572144
COIS- The authors have declared that no competing interests exist.
EDAT- 2008/11/08 09:00
MHDA- 2009/01/06 09:00
PMCR- 2008/11/07
CRDT- 2008/11/08 09:00
PHST- 2008/03/27 00:00 [received]
PHST- 2008/10/09 00:00 [accepted]
PHST- 2008/11/08 09:00 [pubmed]
PHST- 2009/01/06 09:00 [medline]
PHST- 2008/11/08 09:00 [entrez]
PHST- 2008/11/07 00:00 [pmc-release]
AID - 08-PLPA-RA-0294R3 [pii]
AID - 10.1371/journal.ppat.1000198 [doi]
PST - ppublish
SO  - PLoS Pathog. 2008 Nov;4(11):e1000198. doi: 10.1371/journal.ppat.1000198. Epub 
      2008 Nov 7.