PMID- 1898972
OWN - NLM
STAT- MEDLINE
DCOM- 19910219
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 65
IP  - 2
DP  - 1991 Feb
TI  - Differential loss of envelope glycoprotein gp120 from virions of human 
      immunodeficiency virus type 1 isolates: effects on infectivity and 
      neutralization.
PG  - 852-60
AB  - Several parameters which may affect the infectivity of human immunodeficiency 
      virus type 1 in tissue culture were analyzed. In particular, we used gel 
      exclusion chromatography to investigate how the loss of the surface glycoprotein 
      gp120 from virions of the HTLV-IIIB (IIIB), HTLV-IIIRF (RF), and SF-2 isolates 
      modulates infectivity. In IIIB and RF cultures, a high proportion of the total 
      gp120 was virion bound initially but was gradually lost from the virions over 
      time. In contrast, most of the gp120 (and p24) in SF-2-infected cultures was 
      soluble and the few particles present had a fivefold-lower level of virus-bound 
      gp120. However, this reduced level of virion-bound gp120 was more resistant to 
      shedding. Loss of a major proportion of gp120 from IIIB and RF virions resulted 
      in reduced infectivities, and in addition, the resulting accumulation of soluble 
      gp120 in the cultures could competitively inhibit viral infection, especially 
      with SF-2. Increased shedding of virion gp120 also affected the neutralization of 
      IIIB and RF particles. However, the high sensitivity to human serum 
      neutralization characteristic of SF-2 was unaffected by soluble gp120 in 
      cultures, suggesting that the epitopes responsible are not present on soluble 
      gp120.
FAU - McKeating, J A
AU  - McKeating JA
AD  - Chester Beatty Laboratories, Institute of Cancer Research, London, United 
      Kingdom.
FAU - McKnight, A
AU  - McKnight A
FAU - Moore, J P
AU  - Moore JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies)
RN  - 0 (Gene Products, gag)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Viral Core Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies
MH  - Cell Line
MH  - Cell Transformation, Viral
MH  - Chromatography, Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Products, gag/analysis
MH  - HIV Core Protein p24
MH  - HIV Envelope Protein gp120/*analysis/genetics
MH  - HIV Seropositivity
MH  - HIV-1/genetics/immunology/*physiology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neutralization Tests
MH  - Viral Core Proteins/analysis
MH  - Virion/genetics/physiology
MH  - Virus Replication
PMC - PMC239825
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
PMCR- 1991/02/01
CRDT- 1991/02/01 00:00
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
PHST- 1991/02/01 00:00 [entrez]
PHST- 1991/02/01 00:00 [pmc-release]
AID - 10.1128/JVI.65.2.852-860.1991 [doi]
PST - ppublish
SO  - J Virol. 1991 Feb;65(2):852-60. doi: 10.1128/JVI.65.2.852-860.1991.