PMID- 1899008
OWN - NLM
STAT- MEDLINE
DCOM- 19910221
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 260
IP  - 1 Pt 1
DP  - 1991 Jan
TI  - CRF triggers the CNS release of TRH in stress-induced changes in gastric 
      emptying.
PG  - G39-44
AB  - Stress elicited by exposure to cold induces an increase of gastric emptying (GE) 
      and intestinal transit of a caloric meal in mice and the release of 
      corticotropin-releasing factor (CRF) and thyrotropin-releasing hormone (TRH) in 
      the central nervous system (CNS). The present study proposed 1) to compare in 
      mice the central effects of TRH, CRF, and cold-exposure stress on GE and 
      intestinal transit of a caloric test meal consisting of 0.5 ml of reconstituted 
      milk marked with 51Cr-labeled sodium chromate, and 2) using CRF and TRH antisera 
      to determine whether TRH and CRF act in cascade or independently. The 
      intracerebroventricular (icv) injection of TRH (0.5 microgram/kg) and CRF (1 
      microgram/kg), as well as cold stress, significantly increased GE, whereas 
      10-fold higher doses injected intraperitoneally were ineffective. The effect of 
      cold stress on GE was abolished by prior icv injection of both CRF and TRH 
      antisera. The effect of TRH was not blocked by CRF antiserum, but TRH antiserum 
      suppressed the increase in GE induced by CRF. Moreover, both CRF and TRH antisera 
      abolished changes in the rate of GE induced by exogenous CRF and TRH injection, 
      respectively, therefore demonstrating the specific efficiency of 
      immunoneutralization. CRF and cold stress both induced an increase in the rate of 
      intestinal transit, while TRH had no effect. Antibodies to CRF prevented the 
      intestinal stimulatory effect induced by CRF and cold stress. In contrast, 
      antibodies to TRH were unable to antagonize either CRF or cold-stress induced 
      increase in the rate of intestinal transit.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Diop, L
AU  - Diop L
AD  - Department of Pharmacology, Institut National de la Recherche Agronomique, 
      Toulouse, France.
FAU - Pascaud, X
AU  - Pascaud X
FAU - Junien, J L
AU  - Junien JL
FAU - Bueno, L
AU  - Bueno L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Immune Sera)
RN  - 5Y5F15120W (Thyrotropin-Releasing Hormone)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Cerebral Ventricles/drug effects/*physiology
MH  - Cold Temperature
MH  - Corticotropin-Releasing Hormone/administration & dosage/immunology/*pharmacology
MH  - Gastric Emptying/*drug effects
MH  - Gastrointestinal Transit/*drug effects
MH  - Immune Sera/administration & dosage
MH  - Injections, Intraventricular
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Reference Values
MH  - Stress, Physiological/*physiopathology
MH  - Thyrotropin-Releasing Hormone/administration & dosage/immunology/*pharmacology
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1152/ajpgi.1991.260.1.G39 [doi]
PST - ppublish
SO  - Am J Physiol. 1991 Jan;260(1 Pt 1):G39-44. doi: 10.1152/ajpgi.1991.260.1.G39.