PMID- 18990382
OWN - NLM
STAT- MEDLINE
DCOM- 20091103
LR  - 20200816
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 92
IP  - 4
DP  - 2009 Oct
TI  - Application of three-dimensional fluorescence in situ hybridization to human 
      preimplantation genetic diagnosis.
PG  - 1492-1495
LID - S0015-0282(08)03557-7 [pii]
LID - 10.1016/j.fertnstert.2008.08.070 [doi]
AB  - OBJECTIVE: To report a simple and efficient fluorescence in situ hybridization 
      (FISH) method for preimplantation genetic diagnosis (PGD). DESIGN: Technique and 
      method. SETTING: A hospital in vitro fertilization (IVF) laboratory. PATIENT(S): 
      Women undergoing IVF or intracytoplasmic sperm injection (ICSI). INTERVENTION(S): 
      None. MAIN OUTCOME MEASURE(S): The intensity and clarity of signals, technical 
      difficulty, the percentage of successfully treated blastomeres, and blastomere 
      integrity after FISH. RESULT(S): This paraformaldehyde fixation technique 
      simplified the process of fixation of blastomeres for PGD without losing 
      blastomeres during fixation. A total of 35 blastomeres derived from 10 arrested 
      embryos or abnormally fertilized eggs (one pronucleus or three pronuclei) were 
      used for three-dimensional (3D) FISH staining. Signals in all blastomeres were 
      obtained successfully by this method. Approximately 0.1 microL of DNA probe was 
      enough for the detection of the signals in each blastomere, less than the volume 
      (1 microL) used in the conventional FISH. CONCLUSION(S): The 3D-FISH technique 
      for PGD is easy to learn, less damaging to blastomeres, and loses no blastomeres 
      during fixation. It is efficient, feasible, and economic, which allows more 
      patients to benefit from this technique.
FAU - Yan, Li-Ying
AU  - Yan LY
AD  - Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, People's Republic of China.
FAU - Qiao, Jie
AU  - Qiao J
AD  - Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, People's Republic of China. Electronic 
      address: jie.qiao@263.net.
FAU - Chen, Yuan
AU  - Chen Y
AD  - Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, People's Republic of China.
FAU - Huang, Jin
AU  - Huang J
AD  - Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, People's Republic of China.
FAU - Liu, Ping
AU  - Liu P
AD  - Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking 
      University Third Hospital, Beijing, People's Republic of China.
FAU - Sun, Qing-Yuan
AU  - Sun QY
AD  - State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese 
      Academy of Sciences, Beijing, People's Republic of China.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081105
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
SB  - IM
MH  - Blastocyst/cytology
MH  - Blastomeres/cytology
MH  - Cost-Benefit Analysis
MH  - Efficiency
MH  - Feasibility Studies
MH  - Female
MH  - Fertilization in Vitro/methods
MH  - Humans
MH  - Imaging, Three-Dimensional/*methods
MH  - In Situ Hybridization, Fluorescence/economics/*methods
MH  - Pregnancy
MH  - Preimplantation Diagnosis/economics/*methods
EDAT- 2008/11/08 09:00
MHDA- 2009/11/05 06:00
CRDT- 2008/11/08 09:00
PHST- 2008/07/07 00:00 [received]
PHST- 2008/08/04 00:00 [revised]
PHST- 2008/08/06 00:00 [accepted]
PHST- 2008/11/08 09:00 [pubmed]
PHST- 2009/11/05 06:00 [medline]
PHST- 2008/11/08 09:00 [entrez]
AID - S0015-0282(08)03557-7 [pii]
AID - 10.1016/j.fertnstert.2008.08.070 [doi]
PST - ppublish
SO  - Fertil Steril. 2009 Oct;92(4):1492-1495. doi: 10.1016/j.fertnstert.2008.08.070. 
      Epub 2008 Nov 5.