PMID- 18990534
OWN - NLM
STAT- MEDLINE
DCOM- 20090806
LR  - 20151119
IS  - 1879-0070 (Electronic)
IS  - 0732-8893 (Linking)
VI  - 63
IP  - 1
DP  - 2009 Jan
TI  - Repeat-based subtyping and grouping of Staphylococcus aureus from human 
      infections and bovine mastitis using the R-domain of the clumping factor A gene.
PG  - 24-37
LID - 10.1016/j.diagmicrobio.2008.09.004 [doi]
AB  - Staphylococcus aureus has become an emerging public health concern. Markers 
      capable of differentiating separate host-specific lineages are needed for tracing 
      strain sources. Thus, a coding variable number tandem repeat-based typing was 
      explored in this study, based on R-domain of clumping factor A (clfA) gene. DNA 
      from isolates and strains of human infections and bovine mastitis were amplified 
      and sequenced. Sequences of clfA from published strains were also analyzed. 
      Results indicate that except one with 36 copies, 44 of the 55 R-domains had 
      repeat copies between 44 and 57, whereas the remaining 10 had 59.5 to 73 copies. 
      Furthermore, human isolates were polymorphic, while mastitis isolates were 
      clonal. Phylogenetic grouping assigned host-specific strains into respective 
      clusters. The repeats were stable during passages in milk, nutrient broth, and 
      invasion of mammary cells showing suitability for typing. Our data show that the 
      R-domain can be useful for typing and grouping host-specific lineages. Moreover, 
      existence of variant repeats in human strains and the dominance of a clonal motif 
      in mastitis may imply that a specific selection has occurred in the mammary 
      gland.
FAU - Said, Kamaleldin B
AU  - Said KB
AD  - Department of Animal Science, McGill University, Ste Anne de Bellevue, Quebec H9X 
      3V9, Canada.
FAU - Ramotar, Karam
AU  - Ramotar K
FAU - Zhu, Guoqiang
AU  - Zhu G
FAU - Zhao, Xin
AU  - Zhao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081105
PL  - United States
TA  - Diagn Microbiol Infect Dis
JT  - Diagnostic microbiology and infectious disease
JID - 8305899
RN  - 0 (Biomarkers)
RN  - 0 (ClfA protein, Staphylococcus aureus)
RN  - 0 (Coagulase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Biomarkers/analysis
MH  - Cattle
MH  - Cell Line, Transformed
MH  - Coagulase/*genetics
MH  - Community-Acquired Infections/genetics/metabolism
MH  - Humans
MH  - Mastitis, Bovine/*microbiology
MH  - Methicillin-Resistant Staphylococcus aureus/genetics/metabolism
MH  - Milk/metabolism
MH  - Minisatellite Repeats/*genetics
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic/genetics
MH  - Sequence Alignment
MH  - Serial Passage
MH  - Serotyping
MH  - Species Specificity
MH  - Staphylococcal Infections/*microbiology
MH  - Staphylococcus aureus/*genetics/metabolism
EDAT- 2008/11/08 09:00
MHDA- 2009/08/07 09:00
CRDT- 2008/11/08 09:00
PHST- 2008/06/07 00:00 [received]
PHST- 2008/09/04 00:00 [revised]
PHST- 2008/09/11 00:00 [accepted]
PHST- 2008/11/08 09:00 [entrez]
PHST- 2008/11/08 09:00 [pubmed]
PHST- 2009/08/07 09:00 [medline]
AID - S0732-8893(08)00404-5 [pii]
AID - 10.1016/j.diagmicrobio.2008.09.004 [doi]
PST - ppublish
SO  - Diagn Microbiol Infect Dis. 2009 Jan;63(1):24-37. doi: 
      10.1016/j.diagmicrobio.2008.09.004. Epub 2008 Nov 5.