PMID- 18992573
OWN - NLM
STAT- MEDLINE
DCOM- 20090209
LR  - 20181201
IS  - 1097-6841 (Electronic)
IS  - 0022-3913 (Linking)
VI  - 100
IP  - 5
DP  - 2008 Nov
TI  - Effects of provisional acrylic resins on gingival fibroblast cytokine/growth 
      factor expression.
PG  - 390-7
LID - 10.1016/S0022-3913(08)60242-5 [doi]
AB  - STATEMENT OF PROBLEM: Several studies have reported that polymerized resin 
      materials may release agents into surrounding tissues. These agents could alter 
      cytokine/growth factor expression. PURPOSE: The purpose of this study was to 
      determine the effects that provisional acrylic resins have on cell toxicity and 
      the expression of cytokines/growth factors from human gingival fibroblasts 
      (HGFs). MATERIAL AND METHODS: The materials used in this study were chemically 
      activated bis-acryl composite (Chem-Bis), chemically activated polyethyl 
      methacrylate (Chem-PEMA), chemically activated polymethyl methacrylate 
      (Chem-PMMA), and heat-activated polymethyl methacrylate (Heat-PMMA) resins. HGFs 
      were incubated for 72 hours in the presence of eluate from each resin and in the 
      absence of any eluate (negative control). The conditioned media were then 
      collected and stored at -70 degrees C. Cell toxicity was determined using a 
      lactate dehydrogenase method. Cytokine/growth factor expression was examined 
      using cytokine antibody arrays. The experiments were repeated 3 times. The data 
      were analyzed with 1-way ANOVA, Mann-Whitney test, and 1-sample t test 
      (alpha=.05). RESULTS: There was no significant cell toxicity observed from the 
      eluates. The cytokine/growth factor expression induced by Chem-Bis was 
      significantly greater than the control for growth-regulated oncogene (GRO) 
      (P<.001), monocyte chemoattractant protein-1 (MCP-1) (P=.031), and tumor necrosis 
      factor-beta (TNF)-beta (P=.009). For Chem-PEMA, the cytokine/growth factor 
      expression was significantly greater than the control for GRO-alpha (P=.022), 
      interleukin (IL)-13 (P=.031), and TNF-alpha (P=.017). The cytokines/growth 
      factors induced by Chem-PEMA were significantly less than the control (P=.008) 
      and Chem-Bis for IL-8 (P=.042). The expression induced by Chem-PMMA was 
      significantly greater than the control for IL-13 (P=.036), IL-1 alpha (P=.003), 
      IL-2 (P=.020), and IL-5 (P=.045). Finally, Heat-PMMA induced significantly 
      greater levels than the control for GRO (P<.001) and IL-13 (P=.008). CONCLUSIONS: 
      This study demonstrated that the resins evaluated were nontoxic to the HGFs. 
      There were changes in the cytokine/growth factor levels that were statistically 
      significant, but may not be clinically significant.
FAU - Labban, Nawaf
AU  - Labban N
AD  - Department of Restorative Dentistry-Prosthodontics, Indiana University School of 
      Dentistry, Indianapolis, Indiana 46202, USA.
FAU - Song, Fengyu
AU  - Song F
FAU - Al-Shibani, Nouf
AU  - Al-Shibani N
FAU - Windsor, L Jack
AU  - Windsor LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Prosthet Dent
JT  - The Journal of prosthetic dentistry
JID - 0376364
RN  - 0 (Acrylic Resins)
RN  - 0 (Composite Resins)
RN  - 0 (Cytokines)
RN  - 0 (Growth Substances)
RN  - 0 (Polymethacrylic Acids)
RN  - 39341-43-0 (vinyl ethyl methacrylate)
RN  - 9011-14-7 (Polymethyl Methacrylate)
SB  - IM
MH  - Acrylic Resins/*toxicity
MH  - Cells, Cultured
MH  - Composite Resins/toxicity
MH  - Cytokines/biosynthesis
MH  - Dental Restoration, Temporary/*adverse effects
MH  - Fibroblasts/drug effects/metabolism
MH  - Gingiva/cytology/*drug effects/metabolism
MH  - Growth Substances/biosynthesis
MH  - Humans
MH  - Light-Curing of Dental Adhesives
MH  - Polymethacrylic Acids/toxicity
MH  - Polymethyl Methacrylate/toxicity
MH  - Self-Curing of Dental Resins
EDAT- 2008/11/11 09:00
MHDA- 2009/02/10 09:00
CRDT- 2008/11/11 09:00
PHST- 2008/11/11 09:00 [pubmed]
PHST- 2009/02/10 09:00 [medline]
PHST- 2008/11/11 09:00 [entrez]
AID - S0022-3913(08)60242-5 [pii]
AID - 10.1016/S0022-3913(08)60242-5 [doi]
PST - ppublish
SO  - J Prosthet Dent. 2008 Nov;100(5):390-7. doi: 10.1016/S0022-3913(08)60242-5.