PMID- 18992937 OWN - NLM STAT- MEDLINE DCOM- 20090204 LR - 20131121 IS - 1878-5905 (Electronic) IS - 0142-9612 (Linking) VI - 30 IP - 4 DP - 2009 Feb TI - Surface coating of bone marrow cells with N-acetylglucosamine for bone marrow implantation therapy. PG - 574-82 LID - 10.1016/j.biomaterials.2008.10.002 [doi] AB - Bone marrow implantation (BMI) has been performed clinically for the treatment of ischemic cardiovascular diseases. To achieve BMI effectively, accumulation of many bone marrow cells (BMCs) in an infarcted area of the myocardium is important. Previously, we reported that cardiomyocytes show strong interaction with N-acetylglucosamine (GlcNAc) and they can take up GlcNAc-conjugated liposomes. Thus, we examined whether GlcNAc-coated BMCs exhibit strong interaction with cardiomyocytes. The cell surface of BMCs was coated with GlcNAc without causing cell injury by GlcNAc-lipophilic polymers. It was found that the GlcNAc-coated BMCs exhibited strong interaction with cardiomyocytes. At 7 days of coculturing the GlcNAc-coated BMCs with cardiomyocytes, BMC-derived cardiomyocytes were generated. The number of BMC-derived cardiomyocytes was higher following coculture with GlcNAc-coated BMCs than following coculture with uncoated and maltose (MA)-coated BMCs. In this study, we demonstrated that the surface coating of BMCs with GlcNAc can be performed easily by using GlcNAc-lipophilic polymers and that GlcNAc-coated BMCs exhibited strong interaction with cardiomyocytes. Therefore, we think that cell surface coating with GlcNAc would help promote accumulation of BMCs in the infarcted area of the myocardium and that this accumulation would be helpful in the treatment of ischemic cardiovascular diseases with BMI. FAU - Kobayashi, Satoshi AU - Kobayashi S AD - Division of Cardiovascular Medicine, Department of Organ Regeneration, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan. FAU - Ise, Hirohiko AU - Ise H FAU - Takahashi, Masafumi AU - Takahashi M FAU - Goto, Mitsuaki AU - Goto M FAU - Akaike, Toshihiro AU - Akaike T FAU - Ikeda, Uichi AU - Ikeda U LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20081106 PL - Netherlands TA - Biomaterials JT - Biomaterials JID - 8100316 RN - 0 (Coated Materials, Biocompatible) RN - 0 (Glycosides) RN - 0 (Polymers) RN - V956696549 (Acetylglucosamine) SB - IM MH - Acetylglucosamine/*pharmacology MH - Animals MH - Bone Marrow Cells/*cytology/*drug effects MH - *Bone Marrow Transplantation MH - Cell Communication/drug effects MH - Cell Death/drug effects MH - Cells, Cultured MH - Coated Materials, Biocompatible/*pharmacology MH - Coculture Techniques MH - Female MH - Flow Cytometry MH - Glycosides/pharmacology MH - Microscopy, Confocal MH - Myocytes, Cardiac/cytology/drug effects MH - Polymers MH - Rats MH - Rats, Sprague-Dawley MH - Surface Properties EDAT- 2008/11/11 09:00 MHDA- 2009/02/05 09:00 CRDT- 2008/11/11 09:00 PHST- 2008/08/20 00:00 [received] PHST- 2008/10/02 00:00 [accepted] PHST- 2008/11/11 09:00 [pubmed] PHST- 2009/02/05 09:00 [medline] PHST- 2008/11/11 09:00 [entrez] AID - S0142-9612(08)00780-1 [pii] AID - 10.1016/j.biomaterials.2008.10.002 [doi] PST - ppublish SO - Biomaterials. 2009 Feb;30(4):574-82. doi: 10.1016/j.biomaterials.2008.10.002. Epub 2008 Nov 6.