PMID- 18993075
OWN - NLM
STAT- MEDLINE
DCOM- 20090127
LR  - 20131121
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 18
IP  - 22
DP  - 2008 Nov 25
TI  - Evidence for insertional RNA editing in humans.
PG  - 1760-5
LID - 10.1016/j.cub.2008.09.059 [doi]
AB  - Large-scale analysis directly at the protein level holds the promise of 
      uncovering features not apparent or present at the gene level [1-3]. Although 
      mass spectrometry (MS)-based proteomics can now identify and quantify thousands 
      of cellular proteins in large-scale proteomics experiments, much of the peptide 
      information contained in these experiments remains unassigned [4]. Here, we use 
      such information to discover a previously unreported mechanism creating altered 
      protein forms. Linker histones H1 and high-mobility group (HMG) proteins are 
      abundant nuclear proteins that regulate gene expression through modulation of 
      chromatin structure [5-8]. In the high-resolution MS analysis of histone H1 and 
      HMG protein fractions isolated from human cells, we discovered peptides that 
      mapped upstream of the known translation start sites of these genes. No 
      alternative upstream start site exists in the genome, but analysis of Expressed 
      Sequence Tag (EST) databases revealed that these N-terminally extended (ET) 
      proteins are due to in-frame translation of the 5' untranslated region (5'UTR) 
      sequences of the transcripts. The new translation start sites are created by a 
      single uridine insertion between AG, reflecting a previously unreported 
      RNA-editing mechanism. To our knowledge, this is the first report of 
      RNA-insertion editing in humans and may be an example of the type of discoveries 
      possible with modern proteomics methods.
FAU - Zougman, Alexandre
AU  - Zougman A
AD  - Department of Proteomics and Signal Transduction, Max Planck Institute for 
      Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany.
FAU - Ziolkowski, Piotr
AU  - Ziolkowski P
FAU - Mann, Matthias
AU  - Mann M
FAU - Wisniewski, Jacek R
AU  - Wisniewski JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081106
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (5' Untranslated Regions)
RN  - 63231-63-0 (RNA)
RN  - WHI7HQ7H85 (Uridine)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Expressed Sequence Tags
MH  - Genome, Human
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Protein Biosynthesis/physiology
MH  - Proteomics
MH  - RNA/chemistry
MH  - RNA Editing/*physiology
MH  - Sequence Analysis, RNA
MH  - Uridine/chemistry/genetics
EDAT- 2008/11/11 09:00
MHDA- 2009/01/28 09:00
CRDT- 2008/11/11 09:00
PHST- 2008/06/27 00:00 [received]
PHST- 2008/08/28 00:00 [revised]
PHST- 2008/09/19 00:00 [accepted]
PHST- 2008/11/11 09:00 [pubmed]
PHST- 2009/01/28 09:00 [medline]
PHST- 2008/11/11 09:00 [entrez]
AID - S0960-9822(08)01294-3 [pii]
AID - 10.1016/j.cub.2008.09.059 [doi]
PST - ppublish
SO  - Curr Biol. 2008 Nov 25;18(22):1760-5. doi: 10.1016/j.cub.2008.09.059. Epub 2008 
      Nov 6.