PMID- 1899581
OWN - NLM
STAT- MEDLINE
DCOM- 19910321
LR  - 20190820
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 6
IP  - 2
DP  - 1991 Feb
TI  - Modulation of a cloned mouse brain potassium channel.
PG  - 227-36
AB  - The mouse brain K+ channel (MBK), previously cloned by others, has been 
      independently cloned and shown to express in Xenopus oocytes. This K+ current 
      (IK) inactivated over a time course of seconds and was sensitive to the K+ 
      channel-blocking reagent tetraethylammonium. When the K+ channel was coexpressed 
      with a cloned mouse brain serotonin receptor (5HT1c) in oocytes, activation of 
      the 5HT1c receptor by a brief application of serotonin resulted in a suppression 
      of the IK amplitude over the next 20 min. IK could also be suppressed by 
      activation of G proteins. Suppression was also caused by intracellular Ca2+ 
      injections and was blocked by intracellular injection of EGTA. Calmodulin 
      antagonists block the IK suppression, but a known protein kinase inhibitor did 
      not block suppression. The 5HT1c suppression was reversible; recovery from 
      suppression was blocked by the protein kinase inhibitor H-7. These data suggest 
      that the IK suppression occurs through a novel mechanism independent of A- or 
      C-type protein kinases; suppression is best explained as being due to the action 
      of a Ca2+/calmodulin-activated phosphatase; recovery from suppression is due to 
      the action of a protein kinase.
FAU - Hoger, J H
AU  - Hoger JH
AD  - Division of Chemistry and Chemical Engineering, California Institute of 
      Technology, Pasadena 91125.
FAU - Walter, A E
AU  - Walter AE
FAU - Vance, D
AU  - Vance D
FAU - Yu, L
AU  - Yu L
FAU - Lester, H A
AU  - Lester HA
FAU - Davidson, N
AU  - Davidson N
LA  - eng
GR  - BM-10881/BM/FDA HHS/United States
GR  - GM-29836/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (Nucleotides, Cyclic)
RN  - 0 (Potassium Channels)
RN  - 0 (Receptors, Serotonin)
RN  - 333DO1RDJY (Serotonin)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Brain/*metabolism
MH  - Calcium/metabolism/physiology
MH  - *Cloning, Molecular
MH  - Electrophysiology
MH  - Enzyme Activation
MH  - GTP-Binding Proteins/physiology
MH  - Intracellular Membranes/metabolism
MH  - Mice
MH  - Nucleotides, Cyclic/physiology
MH  - Oocytes/metabolism
MH  - Potassium Channels/*genetics/metabolism/physiology
MH  - Protein Kinase C/metabolism
MH  - Protein Kinases/physiology
MH  - Receptors, Serotonin/physiology
MH  - Serotonin/pharmacology
MH  - Xenopus laevis
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
CRDT- 1991/02/01 00:00
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
PHST- 1991/02/01 00:00 [entrez]
AID - 0896-6273(91)90358-7 [pii]
AID - 10.1016/0896-6273(91)90358-7 [doi]
PST - ppublish
SO  - Neuron. 1991 Feb;6(2):227-36. doi: 10.1016/0896-6273(91)90358-7.