PMID- 18996214
OWN - NLM
STAT- MEDLINE
DCOM- 20090330
LR  - 20181201
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 33
IP  - 2
DP  - 2009 Feb
TI  - Multiple shRNA-mediated knockdown of TACE reduces the malignancy of HeLa cells.
PG  - 158-64
LID - 10.1016/j.cellbi.2008.10.008 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) converting enzyme (TACE) is a key enzyme 
      involved in the proteolytic shedding of the ectodomain of several membrane-bound 
      growth factors, cytokines and receptors. Here, we constructed a multiple short 
      hairpin RNA (shRNA) expression vector containing four shRNAs against TACE. We 
      found that in HeLa cells our multiple shRNAs vector produced a higher level of 
      TACE knockdown than any single shRNA vector containing only one TACE shRNA. 
      Silencing TACE expression in HeLa cells decreased their malignancy by decreasing 
      the proliferation, adhesion and migration, as well as inducing apoptosis in these 
      cells. Furthermore, our data suggest that the effects of TACE on the malignancy 
      of HeLa cells may be mediated via activation of the EGFR (epidermal growth factor 
      receptor) signaling pathway. Our findings suggest that using a combination of 
      shRNAs within one vector to silence the expression of TACE might be a potential 
      therapeutic strategy for tumors.
FAU - Yan, Yuan
AU  - Yan Y
AD  - Department of Biochemistry and Molecular Biology, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan 430030, PR China.
FAU - Zhang, Jie
AU  - Zhang J
FAU - Guo, Jian-Li
AU  - Guo JL
FAU - Huang, Wei
AU  - Huang W
FAU - Yang, Yu-Zhen
AU  - Yang YZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081101
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (RNA, Untranslated)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.86 (ADAM17 Protein)
RN  - EC 3.4.24.86 (ADAM17 protein, human)
SB  - IM
MH  - ADAM Proteins/*antagonists & inhibitors/genetics/metabolism
MH  - ADAM17 Protein
MH  - Apoptosis
MH  - ErbB Receptors/metabolism
MH  - Gene Knockdown Techniques
MH  - HeLa Cells
MH  - Humans
MH  - Neoplasms/*therapy
MH  - *RNA Interference
MH  - RNA, Untranslated/chemistry
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2008/11/11 09:00
MHDA- 2009/03/31 09:00
CRDT- 2008/11/11 09:00
PHST- 2008/05/26 00:00 [received]
PHST- 2008/08/13 00:00 [revised]
PHST- 2008/10/13 00:00 [accepted]
PHST- 2008/11/11 09:00 [pubmed]
PHST- 2009/03/31 09:00 [medline]
PHST- 2008/11/11 09:00 [entrez]
AID - S1065-6995(08)00601-X [pii]
AID - 10.1016/j.cellbi.2008.10.008 [doi]
PST - ppublish
SO  - Cell Biol Int. 2009 Feb;33(2):158-64. doi: 10.1016/j.cellbi.2008.10.008. Epub 
      2008 Nov 1.