PMID- 18996346
OWN - NLM
STAT- MEDLINE
DCOM- 20081126
LR  - 20200930
IS  - 1934-6069 (Electronic)
IS  - 1931-3128 (Print)
IS  - 1931-3128 (Linking)
VI  - 4
IP  - 5
DP  - 2008 Nov 13
TI  - Autophagosome-independent essential function for the autophagy protein Atg5 in 
      cellular immunity to intracellular pathogens.
PG  - 458-69
LID - 10.1016/j.chom.2008.10.003 [doi]
AB  - The physiologic importance of autophagy proteins for control of mammalian 
      bacterial and parasitic infection in vivo is unknown. Using mice with 
      granulocyte- and macrophage-specific deletion of the essential autophagy protein 
      Atg5, we show that Atg5 is required for in vivo resistance to the intracellular 
      pathogens Listeria monocytogenes and Toxoplasma gondii. In primary macrophages, 
      Atg5 was required for interferongamma (IFN-gamma)/LPS-induced damage to the T. 
      gondii parasitophorous vacuole membrane and parasite clearance. While we did not 
      detect classical hallmarks of autophagy, such as autophagosomes enveloping T. 
      gondii, Atg5 was required for recruitment of IFN-gamma-inducible p47 GTPase IIGP1 
      (Irga6) to the vacuole membrane, an event that mediates IFN-gamma-mediated 
      clearance of T. gondii. This work shows that Atg5 expression in phagocytic cells 
      is essential for cellular immunity to intracellular pathogens in vivo, and that 
      an autophagy protein can participate in immunity and intracellular killing of 
      pathogens via autophagosome-independent processes such as GTPase trafficking.
FAU - Zhao, Zijiang
AU  - Zhao Z
AD  - Department of Pathology and Immunology, Washington University School of Medicine, 
      St. Louis, MO 63110, USA.
FAU - Fux, Blima
AU  - Fux B
FAU - Goodwin, Megan
AU  - Goodwin M
FAU - Dunay, Ildiko R
AU  - Dunay IR
FAU - Strong, David
AU  - Strong D
FAU - Miller, Brian C
AU  - Miller BC
FAU - Cadwell, Ken
AU  - Cadwell K
FAU - Delgado, Monica A
AU  - Delgado MA
FAU - Ponpuak, Marisa
AU  - Ponpuak M
FAU - Green, Karen G
AU  - Green KG
FAU - Schmidt, Robert E
AU  - Schmidt RE
FAU - Mizushima, Noboru
AU  - Mizushima N
FAU - Deretic, Vojo
AU  - Deretic V
FAU - Sibley, L David
AU  - Sibley LD
FAU - Virgin, Herbert W
AU  - Virgin HW
LA  - eng
GR  - R01 AI036629/AI/NIAID NIH HHS/United States
GR  - R01 AI042999/AI/NIAID NIH HHS/United States
GR  - AI071299/AI/NIAID NIH HHS/United States
GR  - U54 AI057160-010005/AI/NIAID NIH HHS/United States
GR  - R01 AI069345/AI/NIAID NIH HHS/United States
GR  - R37 AI042999/AI/NIAID NIH HHS/United States
GR  - AI036629/AI/NIAID NIH HHS/United States
GR  - U54 AI057160/AI/NIAID NIH HHS/United States
GR  - R21 AI071299/AI/NIAID NIH HHS/United States
GR  - R21 AI071299-02/AI/NIAID NIH HHS/United States
GR  - AI069345/AI/NIAID NIH HHS/United States
GR  - R01 AI036629-15/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Host Microbe
JT  - Cell host & microbe
JID - 101302316
RN  - 0 (Atg5 protein, mouse)
RN  - 0 (Autophagy-Related Protein 5)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
CIN - Cell Host Microbe. 2008 Nov 13;4(5):413-4. PMID: 18996340
MH  - Animals
MH  - *Autophagy
MH  - Autophagy-Related Protein 5
MH  - Cells, Cultured
MH  - *Host-Pathogen Interactions
MH  - Humans
MH  - Immunity, Cellular
MH  - Interferon-gamma/immunology
MH  - Listeria monocytogenes/*physiology
MH  - Listeriosis/*immunology/microbiology
MH  - Lysosomes/immunology/parasitology
MH  - Macrophages/*immunology/parasitology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Mice, Knockout
MH  - Microtubule-Associated Proteins/genetics/*immunology
MH  - Signal Transduction
MH  - Toxoplasma/*physiology
MH  - Toxoplasmosis/*immunology/parasitology
PMC - PMC2682425
MID - NIHMS79827
EDAT- 2008/11/11 09:00
MHDA- 2008/12/17 09:00
PMCR- 2008/11/12
CRDT- 2008/11/11 09:00
PHST- 2008/06/01 00:00 [received]
PHST- 2008/08/29 00:00 [revised]
PHST- 2008/10/07 00:00 [accepted]
PHST- 2008/11/11 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/11/11 09:00 [entrez]
PHST- 2008/11/12 00:00 [pmc-release]
AID - S1931-3128(08)00329-6 [pii]
AID - 10.1016/j.chom.2008.10.003 [doi]
PST - ppublish
SO  - Cell Host Microbe. 2008 Nov 13;4(5):458-69. doi: 10.1016/j.chom.2008.10.003.