PMID- 18997033
OWN - NLM
STAT- MEDLINE
DCOM- 20090126
LR  - 20181113
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 75
IP  - 1
DP  - 2009 Jan
TI  - Abundance and activity of methanotrophic bacteria in littoral and profundal 
      sediments of lake constance (Germany).
PG  - 119-26
LID - 10.1128/AEM.01350-08 [doi]
AB  - The abundances and activities of aerobic methane-oxidizing bacteria (MOB) were 
      compared in depth profiles of littoral and profundal sediments of Lake Constance, 
      Germany. Abundances were determined by quantitative PCR (qPCR) targeting the pmoA 
      gene and by fluorescence in situ hybridization (FISH), and data were compared to 
      methane oxidation rates calculated from high-resolution concentration profiles. 
      qPCR using type I MOB-specific pmoA primers indicated that type I MOB represented 
      a major proportion in both sediments at all depths. FISH indicated that in both 
      sediments, type I MOB outnumbered type II MOB at least fourfold. Results obtained 
      with both techniques indicated that in the littoral sediment, the highest numbers 
      of methanotrophs were found at a depth of 2 to 3 cm, corresponding to the zone of 
      highest methane oxidation activity, although no oxygen could be detected in this 
      zone. In the profundal sediment, highest methane oxidation activities were found 
      at a depth of 1 to 2 cm, while MOB abundance decreased gradually with sediment 
      depth. In both sediments, MOB were also present at high numbers in deeper 
      sediment layers where no methane oxidation activity could be observed.
FAU - Rahalkar, M
AU  - Rahalkar M
AD  - Universitat Konstanz, Fachbereich Biologie, LS Mikrobielle Okologie, Fach M 654, 
      D-78457 Constance, Germany.
FAU - Deutzmann, J
AU  - Deutzmann J
FAU - Schink, B
AU  - Schink B
FAU - Bussmann, I
AU  - Bussmann I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081107
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Primers)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Aerobiosis
MH  - Bacteria/genetics/*isolation & purification/*metabolism
MH  - Bacterial Proteins/genetics
MH  - DNA Primers/genetics
MH  - Geologic Sediments/*microbiology
MH  - Germany
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Methane/*metabolism
MH  - Oxidation-Reduction
MH  - Polymerase Chain Reaction/methods
PMC - PMC2612212
EDAT- 2008/11/11 09:00
MHDA- 2009/01/27 09:00
PMCR- 2009/07/01
CRDT- 2008/11/11 09:00
PHST- 2008/11/11 09:00 [pubmed]
PHST- 2009/01/27 09:00 [medline]
PHST- 2008/11/11 09:00 [entrez]
PHST- 2009/07/01 00:00 [pmc-release]
AID - AEM.01350-08 [pii]
AID - 1350-08 [pii]
AID - 10.1128/AEM.01350-08 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2009 Jan;75(1):119-26. doi: 10.1128/AEM.01350-08. Epub 
      2008 Nov 7.