PMID- 18997155
OWN - NLM
STAT- MEDLINE
DCOM- 20090415
LR  - 20210512
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 50
IP  - 3
DP  - 2009 Mar
TI  - Endothelin-converting enzyme-1 increases in atherosclerotic mice: potential role 
      of oxidized low density lipoproteins.
PG  - 364-375
LID - S0022-2275(20)30880-4 [pii]
LID - 10.1194/jlr.M800215-JLR200 [doi]
AB  - The aim of our study was to analyze the relationships between atherosclerosis and 
      endothelin-converting enzyme-1 (ECE-1). Four-week-old C57BL/6J [wild-type (WT)] 
      and apolipoprotein E-deficient (apoE) mice were fed with a standard or 
      Western-type fat diet for 8 wks. ApoE showed atherosclerotic lesions in the 
      aorta, higher blood pressure and vascular lectin-like oxidized low-density 
      lipoprotein receptor-1 (LOX-1) protein content than WT. ApoE showed a significant 
      increase in ECE-1 protein content and mRNA expression in aorta, lung, and kidney, 
      without changes in heart. When an ECE-1 inhibitor, FR-901533, was administered to 
      them, blood pressure decreased in apoE on fat diet versus apoE on normal diet and 
      WT. ECE-1 and LOX-1 protein content were elevated in peripheral blood mononuclear 
      cells (PBMC) from hypercholesterolemic patients. In order to study the mechanism 
      involved in this ECE-1 up-regulation, bovine aortic endothelial cells (BAEC) were 
      treated with oxidized-low density lipoproteins (oxLDL). OxLDL, but not LDL, 
      increased ECE-1 protein content, mRNA expression and promoter activity. Our 
      results demonstrate that ECE-1 increases in different atherosclerosis situations. 
      Up-regulation of ECE-1 could contribute, at least partially, to the development 
      of hypertension seen in apoE mice, because FR-901533 avoided it. Probably, 
      atherosclerotic situations course with an increase of oxLDL, which is able to 
      induce ECE-1 expression with the subsequent potential pathological effects.
FAU - Martinez-Miguel, Patricia
AU  - Martinez-Miguel P
AD  - P. Martinez-Miguel and V. Raoch contributed equally to the manuscript; Research 
      Unit and Nephrology Section, Hospital Universitario Principe de Asturias, Alcala 
      University, Madrid.
FAU - Raoch, Viviana
AU  - Raoch V
AD  - P. Martinez-Miguel and V. Raoch contributed equally to the manuscript; Research 
      Unit and Nephrology Section, Hospital Universitario Principe de Asturias, Alcala 
      University, Madrid.
FAU - Zaragoza, Carlos
AU  - Zaragoza C
AD  - Centro Nacional de Investigaciones Cardiovascular (CNIC), Madrid, Lerida, Spain.
FAU - Valdivielso, Jose Manuel
AU  - Valdivielso JM
AD  - Hospital Universitario Arnau de Vilanova, Lerida, Spain.
FAU - Rodriguez-Puyol, Manuel
AU  - Rodriguez-Puyol M
AD  - Physiology and Medicine Departments, Alcala University, Madrid.
FAU - Rodriguez-Puyol, Diego
AU  - Rodriguez-Puyol D
AD  - D. Rodriguez-Puyol and S. Lopez-Ongil contributed equally to the manuscript; 
      Research Unit and Nephrology Section, Hospital Universitario Principe de 
      Asturias, Alcala University, Madrid; Physiology and Medicine Departments, Alcala 
      University, Madrid.
FAU - Lopez-Ongil, Susana
AU  - Lopez-Ongil S
AD  - D. Rodriguez-Puyol and S. Lopez-Ongil contributed equally to the manuscript; 
      Research Unit and Nephrology Section, Hospital Universitario Principe de 
      Asturias, Alcala University, Madrid. Electronic address: 
      slopez.hupa@salud.madrid.org.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081107
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Apolipoproteins E)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (NF-kappa B)
RN  - 0 (OLR1 protein, human)
RN  - 0 (Olr1 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Scavenger Receptors, Class E)
RN  - 0 (oxidized low density lipoprotein)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.23.- (Aspartic Acid Endopeptidases)
RN  - EC 3.4.24.- (Metalloendopeptidases)
RN  - EC 3.4.24.71 (ECE1 protein, human)
RN  - EC 3.4.24.71 (Ece1 protein, mouse)
RN  - EC 3.4.24.71 (Endothelin-Converting Enzymes)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Apolipoproteins E/deficiency/genetics
MH  - Aspartic Acid Endopeptidases/genetics/*metabolism
MH  - Atherosclerosis/etiology/genetics/*metabolism/physiopathology
MH  - Base Sequence
MH  - Cattle
MH  - Cells, Cultured
MH  - DNA/genetics
MH  - Endothelial Cells/drug effects/metabolism
MH  - Endothelin-Converting Enzymes
MH  - Humans
MH  - Hypercholesterolemia/blood/enzymology
MH  - Lipoproteins, LDL/*metabolism/pharmacology
MH  - Male
MH  - Metalloendopeptidases/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Middle Aged
MH  - NF-kappa B/metabolism
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics/metabolism
MH  - Scavenger Receptors, Class E/blood/metabolism
MH  - Up-Regulation/drug effects
EDAT- 2008/11/11 09:00
MHDA- 2009/04/16 09:00
CRDT- 2008/11/11 09:00
PHST- 2008/11/11 09:00 [pubmed]
PHST- 2009/04/16 09:00 [medline]
PHST- 2008/11/11 09:00 [entrez]
AID - S0022-2275(20)30880-4 [pii]
AID - 10.1194/jlr.M800215-JLR200 [doi]
PST - ppublish
SO  - J Lipid Res. 2009 Mar;50(3):364-375. doi: 10.1194/jlr.M800215-JLR200. Epub 2008 
      Nov 7.